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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
1. Epidemiological
studies (parasitological, serological and molecular techniques) of Trypanosoma
evansi infection in
camels (Camelus dromedarius) in Egypt -
Ahmed Abdel-Rady
Vet World. 2008; 1(11): 325-328
Abstract
Trypanosomosis in
camel caused by Trypanosoma
evansi is still a
serious problem in camel husbandry causes considerable economic
losses in many camel-rearing regions of the world. In the present
study 193 camels clinically suspected for surra were examined
parasitologically by Giemsa stained blood smear (GSBS) and
haematocrit centrifugation technique, serologically for detection
of anti-trypanosomal antibodies by card agglutination test for
trypanosomes (CATT), and for DNA amplification, by Polymerase
chain reaction (PCR), with primers yielding a 177 bp PCR product
for the specific detection ofTrypanozoon parasites.
Out of 193, eight camels were positive by GSBS (4.1%) while 12
were positive with haematocrit centrifugation technique (6.2%).
Detection of anti-trypanosomal antibodies with CATT yielded 84
positive samples (43.5%). Using PCR 110 out of 193 were positive
(56.9 %). PCR technique is accurate, more sensitive and specific
method for diagnosis of trypanosome infected camels than
parasitological techniques; it overcomes the problem of
specificity and can detect low parasitemic camels in chronic
cases. The PCR proved to be the best test used for detection of
camel trypanosomosis in Egypt.
Keywords: Camels,
Trypanosomosis, Stained Blood smear, Haematocrit centrifugation
technique (HCT), Card agglutination test (CATT), Polymerase chain
reaction (PCR)
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