Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks

Research (Published online: 02-08-2017)

2. Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks

Hazem Mohammed Ibrahim, Rafik Hamed Sayed, Wafaa Ragab Abdel-Aziz and Rafik Tawfik Soliman

Veterinary World, 10(8): 848-853

Hazem Mohammed Ibrahim: Department of Bacterial Sera and Antigen Research, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.

Rafik Hamed Sayed: Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.

Wafaa Ragab Abdel-Aziz: Department of Bacterial Sera and Antigen Research, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.

Rafik Tawfik Soliman: Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.

doi: 10.14202/vetworld.2017.848-853

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Article history: Received: 10-04-2017, Accepted: 22-06-2017, Published online: 02-08-2017

Corresponding author: Hazem Mohammed Ibrahim

E-mail: dr.hazemibrahim@gmail.com

Citation: Ibrahim HM, Sayed RH, Abdel-Aziz WR, Soliman RT (2017) Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks, Veterinary World, 10(8): 848-853.

Abstract

Aim: The present work aimed to develop lateral flow immunochromatographic test (LFIT) for detection of Salmonella Enteritidis (SE) specific antibodies in chicken sera.

Materials and Methods: A rapid LFIT has been developed, in which SE Group D antigen labeled with the gold chloride molecules laid on the conjugate pad. Staphylococcus aureus protein A was used as capture antibody at the test line (T) of a nitrocellulose (NC) membrane and anti-SE antigen-specific rabbit antibodies were used as capture antibody at the control line (C) of the NC strip in the lateral flow layout device.

Results: Using the developed LFIT, the minimal amount of SE-specific antibodies that can be detected in chicken serum sample was 1427 enzyme-linked immunosorbent assay (ELISA) unit/100 μl that was equal to 0.1 μg (Ab)/100 μl sample. 100 suspected serum samples collected from a poultry flock were tested with the prepared SE-LFIT kits and the locally prepared stained Salmonella antigen, and the results were compared with those obtained from examination of these samples with Salmonella Group D antibody ELISA kit as the gold standard test. The sensitivity, specificity, and accuracy of the prepared SE-LFIT antigen kits were 94.4%, 90%, and 94%, respectively, while those obtained with stained Salmonella antigen were 88.8%, 90%, and 89%, respectively.

Conclusion: The developed test is a simple field rapid test of high sensitivity, specificity, and accuracy that can improve and facilitates rapid field surveillance of salmonellosis among chickens.

Keywords: diagnosis, lateral flow, poultry, Salmonella Enteritidis.

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