Open Access
Research
(Published
online: 18-02-2017)
12.
Biochemical components of seminal
plasma and their correlation to the fresh seminal characteristics
in Marwari stallions and Poitou jacks -
Thirumala Rao Talluri,
Gorakh Mal
and Sanjay Kumar Ravi
Veterinary World, 10(2): 214-220
doi:
10.14202/vetworld.2017.214-220
Thirumala Rao
Talluri :
Department of
Animal Reproduction, Equine Production Campus, Indian Council of
Agricultural Research-National Research Centre on Equines,
Bikaner, Rajasthan-334001, India.
Gorakh Mal :
Department of
Biochemistry, Biochemistry Laboratory, Indian Council of
Agricultural Research - Indian Veterinary Research Institute,
Palampur - 176 061, Himachal Pradesh, India.
Sanjay Kumar Ravi :
Department of
Animal Reproduction, Equine Production Campus, Indian Council of
Agricultural Research-National Research Centre on Equines, Bikaner,
Rajasthan-334001, India.
Received: 24-10-2016, Accepted: 18-01-2017, Published online:
18-02-2017
Corresponding author:
Thirumala Rao
Talluri, e-mail: raotalluri98@gmail.com
Citation:
Talluri TR, Mal G, Ravi SK (2017) Biochemical components of
seminal plasma and their correlation to the fresh seminal
characteristics in Marwari stallions and Poitou jacks,
Veterinary World, 10(2): 214-220.
Abstract
Aim:
To investigate
various biochemical components of seminal plasma in Marwari
stallions and Poitou Jacks and to find out their correlation with
that of the seminal characteristics.
Materials and
Methods:
In this study,
semen was collected from six Marwari stallions and six Poitou
jacks aged from 4 to 6 years and with known fertility status. The
semen collection from the stallions were collected during the
breeding season, i.e., between the months of April and June. From
the collected semen ejaculates, we estimated the values of some
biochemical components, viz., total protein content, total lipid
content, and enzymes such as glutamic pyruvic transaminase (GPT),
glutamic oxaloacetic transaminase (GOT), alkaline phosphatase
(ALP), acid phosphatase (ACP), and lactate dehydrogenase (LDH) as
well as concentrations of glucose, cholesterol, total calcium
(Ca), and phosphorus (P) and correlations among different seminal
parameters were statistically examined using the Pearson
correlation coefficient.
Results:
In this study,
we found positive correlations between semen volume as well as
sperm concentration and GOT, GPT, ALP and ACP for both the group
stallions. Significant correlation between motility and glucose,
GOT and GPT could be an indication for their role metabolism and
protection against free radicals to the spermatozoa.
Conclusion:
Based
on the results, it is concluded that there is a positive
correlation between some biochemical values such as glucose, Ca,
ALP, and LDH and seminal parameters which play a key role in
capacitation and onward movement of the spermatozoa.
Keywords:
alkaline phosphatase, biochemical components, lactate-dehydrogenase,
Marwari, Poitou, stallion, seminal plasma.
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