Open Access
Research
(Published
online: 21-02-2017)
15.
Potential antibacterial activity of
some Saudi Arabia honey -
Ahmed G. Hegazi, Faiz M. Al Guthami, Ahmed F. M. Al Gethami,
Fyrouz M. Abd Allah, Ashraf A. Saleh
and Ehab A. Fouad
Veterinary World, 10(2): 233-237
doi:
10.14202/vetworld.2017.233-237
Ahmed G. Hegazi :
Department of
Zoonotic Diseases, National Research Centre, Dokki, Giza, Egypt.
Faiz M. Al Guthami :
Al Guthami
foundation, Saudi Arabia.
Ahmed F. M. Al
Gethami :
Al Guthami
foundation, Saudi Arabia.
Fyrouz M. Abd Allah :
Department of
Parasitology, National Research Centre, Dokki, Giza, Egypt.
Ashraf A. Saleh :
Department of
Parasitology, National Research Centre, Dokki, Giza, Egypt.
Ehab A. Fouad :
Department of
Microbiology and Immunology, National Research Centre, Dokki, Giza,
Egypt.
Received: 21-09-2016, Accepted: 17-01-2017, Published online:
21-02-2017
Corresponding author:
Ahmed G. Hegazi,
e-mail: ahmedhegazi128@gmail.com
Citation:
Hegazi AG, Al Guthami FM, Al Gethami AFM, Allah FMA, Saleh AA,
Fouad EA (2017) Potential antibacterial activity of some Saudi
Arabia honey, Veterinary World, 10(2): 233-237.
Abstract
Aim:
The aim of
this study was to investigate the potential antibacterial activity
of some Saudi Arabia honey against selected bacterial strains of
medical importance.
Materials and
Methods:
A total of 10
Saudi Arabia honey used to evaluate their antimicrobial activity
against some antibiotic-resistant pathogenic bacterial strains.
The bacterial strains were Staphylococcus aureus, Streptococcus
pyogenes, Klebsiella pneumoniae, Escherichia coli, and
Pseudomonas aeruginosa.
Results:
The
antibacterial activity of Saudi honey against five bacterial
strains showed different levels of inhibition according to the
type of honey. The overall results showed that the potential
activity was differing according to the pathogen and honey type.
Conclusion:
It
could be concluded that the Saudi honey inhibit the growth of
bacterial strains and that honey can be used as complementary
antimicrobial agent against selected pathogenic bacteria.
Keywords:
antibiotic-resistant, potential antibacterial activity, Saudi
Arabia honey.
References
1. Wright,
G.D. (2007) The antibiotic resisted: The nexus of chemical and
genetic diversity. Nat. Rev. Microbiol., 5: 175-186.
https://doi.org/10.1038/nrmicro1614
PMid:17277795 |
|
2. Byarugaba,
D.K. (2009) Mechanisms of antimicrobial resistance cited. In:
Sosa, A.D.J., Byarugaba, D.K., Amabile-Cuevas, C.F., Hsueh,
P.R., Kariuki, S. and Okeke, I.N., editors. Antimicrobial
Resistance in Developing Countries. Ch. 2. Springer Science +
Business Media, LLC. p15-16. DOI: 10.1007/978-0-387-89370-9_2.
https://doi.org/10.1007/978-0-387-89370-9_2 |
|
3. Ayaad,
T.H., Shaker, G.H. and Almuhnaa, A.M. (2009) Isolation of
antibacterial peptides from Saudi Arabian honeybees and
investigating the antimicrobial properties of natural honey
samples. Egypt. Acad. J. Biol. Sci., 2(2): 23-34. |
|
4.
Alvarez-Suarez, J.M., Tulipani, S., Romandini, S., Bertoli, E.
and Battino, M. (2010) Contribution of honey in nutrition and
human health: A review. Med. J. Nutr. Metab., 3: 15-23.
https://doi.org/10.1007/s12349-009-0051-6 |
|
5.
Wilkinson, J.M. and Cavanagh, H.M. (2005) Antibacterial
activity of 13 honeys against Escherichia coli and Pseudomonas
aeruginosa. J. Med. Food, 2: 100-103.
https://doi.org/10.1089/jmf.2005.8.100
PMid:15857217 |
|
6. Chute,
R.K., Deogade, N.G. and Kawale, M. (2010) Antimicrobial
activity of Indian honey against clinical isolates. Asiatic J.
Biotechnol. Resour., 1: 35-38. |
|
7. Hegazi,
A.G. (2011) Antimicrobial activity of different Egyptian
honeys as comparison of Saudi Arabia Hone. Res. J. Microbiol.,
6(5): 488-495.
https://doi.org/10.3923/jm.2011.488.495 |
|
8. Hegazi,
A.G. and Abd Allah, F.M. (2012) Antimicrobial activity of
different Saudi Arabia honeys. Glob. Vet., 9(1): 53-59. |
|
9. Toussoun,
Z., Rashed, A. and Hegazi, A.G. (1997) Honey and propolis as
management of some chronic skin ulcers. Proceeding of
International Symposium on Apitherapy, Cairo 8-9th, March,
1997. p71. |
|
10.
Subrahmanyam, M., Hemmady, A. and Pawar, S.G. (2001)
Antibacterial activity of honey on bacteria isolated from
wounds. Ann. Burns Fire Disasters, 14: 198-201. |
|
11.
Brudzynski, K. (2006) Effect of hydrogen peroxide on
antibacterial activities of Canadian honeys. Can. J. Microbiol.,
52: 1228-1237.
https://doi.org/10.1139/w06-086
PMid:17473892 |
|
12. Fakoor,
M. and Pipelzadeh, M.H. (2007) A study on the healing effect
of honey on infected open fracture wounds. Pak. J. Med. Sci.,
23: 327-329. |
|
13. Tumin,
N., Halim, N.A., Shahjahan, M., Noor, I.N., Sattar, M.A.,
Khan, A.H. and Mohsin, S.S. (2005) Antibacterial activity of
local Malaysian honey. Malays. J. Pharm. Sci., 3: 1-10. |
|
14. Kumar,
K.P., Bhowmik, D., Biswajit, C. and Chandira, M.R. (2010)
Medicinal uses and health benefits of honey: An overview. J.
Chem. Pharm. Res., 2(1): 385-395. |
|
15.
Eteraf-Oskouei, T. and Najafi, M. (2013) Traditional and
modern uses of natural honey in human diseases: A review.
Iran. J. Basic Med. Sci., 16(6): 731-742.
PMid:23997898 PMCid:PMC3758027 |
|
16. Hussain,
M.B., Hannan, A., Akhtar, N., Fayyaz, Q.G., Imran, M., Saleem,
S. and Qureshi, I.A. (2015) Evaluation of the antibacterial
activity of selected Pakistani honeys against multi-drug
resistant Salmonella typhi. BMC Complement. Altern. Med., 15:
32.
https://doi.org/10.1186/s12906-015-0549-z
PMid:25880671 PMCid:PMC4355501 |
|
17. Halawani,
E.M. (2006) A study on Salmonella typhimurium causing food
poisoning in Al-Taif city and antibacterial effect of Nigella
sativa honey and Camels urine. Ph.D Thesis. Taif University,
Saudi Arabia. |
|
18. Ajibola,
A., Chamunorwa, J.P. and Erlwanger, K.H. (2012) Nutraceutical
values of natural honey and its contribution to human health
and wealth. Nutr. Metab., 9: 61.
https://doi.org/10.1186/1743-7075-9-61
PMid:22716101 PMCid:PMC3583289 |
|
19. Molan,
P. (2002) Not all honeys are the same for wound healing. Bull.
Eur. Tissue Rep. Soc., 9: 5-6. |
|
20. Molan,
P.C. (1992) The antibacterial activity of honey. 1. The nature
of the antibacterial activity. Bee World, 73: 5-28.
https://doi.org/10.1080/0005772X.1992.11099109 |
|
21. Kwakman,
P.H., Te Velde, A.A., De Boer, L., Speijer, D.V.,
Enbroucke-Grauls, C.M. and Zaat, S.A. (2010) How honey kills
bacteria. FASEB J., 24: 2576-2582.
https://doi.org/10.1096/fj.09-150789
PMid:20228250 |
|
22. Mercan,
N., Guvensen, A., Celik, A. and Katircioglu, H. (2007)
Antimicrobial activity and pollen composition of honey samples
collected from different provinces in Turkey. Nat. Prod. Res.,
21(3): 187-195.
https://doi.org/10.1080/14786410600906277
PMid:17365707 |
|
23. Nour,
M.E. (1988) Some Factors Affecting Quality of Egyptian Honeys.
Ph. D. Thesis. Faculty of Agriculture, Cairo University. |
|
24. Irish,
J., Blair, S. and Carter, D.A. (2011) The antibacterial
activity of honey derived from Australian Flora. Otto M, ed.
PLoS One, 6(3): e18229.
https://doi.org/10.1371/journal.pone.0018229 |
|
25. Watt,
B.E., Proudfoot, A.T. and Vale, J.A. (2004) Hydrogen peroxide
poisoning. Toxicol. Rev., 23: 51-57.
https://doi.org/10.2165/00139709-200423010-00006
PMid:15298493 |
|
26. Cooper,
R.A., Molan, P.C. and Harding, K.G. (2002) The sensitivity to
honey of gram-positive cocci of clinical significance isolated
from wounds. J. Appl. Microbiol., 93: 857-863.
https://doi.org/10.1046/j.1365-2672.2002.01761.x
PMid:12392533 |
|
27. Mandal,
M.D. and Mandal, S. (2011) Honey: Its medicinal property and
antibacterial activity. Asian Pac. J. Trop. Biomed., 1(2):
154-160.
https://doi.org/10.1016/S2221-1691(11)60016-6 |
|
28.
Moniruzzaman, M., Sulaiman, S.A., Khalil, M.I. and Gan, S.H.
(2013) Evaluation of physicochemical and antioxidant
properties of sourwood and other Malaysian honeys: A
comparison with Manuka honey. Chem. Cent. J., 7: 138.
https://doi.org/10.1186/1752-153x-7-138 |
|
29. Yao, L.,
Jiang, Y., Singanusong, R., Datta, N. and Raymont, K. (2004)
Phenolic acids and abscisic acid in Australian Eucalyptus
honeys and their potential for floral authentication. Food
Chem., 86: 169-177.
https://doi.org/10.1016/j.foodchem.2003.08.013 |
|
30. Mavric,
E., Wittmann, S., Barth, G. and Henle, T. (2008)
Identification and quantification of methylglyoxal as the
dominant antibacterial constituent of manuka (Leptospermum
scoparium) honeys from New Zealand. Mol. Nutr. Food Res.,
52(4): 483-489.
https://doi.org/10.1002/mnfr.200700282
PMid:18210383 |
|
31. Tonks,
A., Cooper, R.A., Price, A.J., Molan, P.C. and Jones, K.P.
(2001) Stimulation of TNF-alpha release in monocytes by honey.
Cytokine, 14: 240-242.
https://doi.org/10.1006/cyto.2001.0868
PMid:11448125 |
|
32. Tonks,
A.J., Cooper, R.A., Jones, K.P., Blair, S.J., Parton, G.H. and
Tonks, A. (2003) Honey stimulates inflammatory cytokine
production from monocytes. Cytokine, 17: 21-23.
https://doi.org/10.1016/s1043-4666(03)00092-9 |
|
33. Molan,
P.C., Coley, K.E., Alsomal, N. and Hancock, B.M. (1994)
Susceptibility of Helicobacter pylori to the antibacterial
activity of Manuka honey. J. R. Soc. Med., 87: 9-12.
PMid:8308841 PMCid:PMC1294271 |
|
34. Hegazi,
A.G., Moharm, N.Z., Abd Allah, F., Nour, M.S. and Khair, A.M.
(2002) Antibacterial activity of different Egyptian honeys in
relation to some bee products. Egypt. J. Vet. Sci., 36: 31-42. |
|
35. Halawani,
E.M. and Shohayeb, M.M. (2011a) Shaoka and Sidr honeys surpass
in their antibacterial activity local and imported honeys
available in Saudi markets against pathogenic and food
spoilage bacteria. Aust. J. Basic Appl. Sci., 5(4): 187-191. |
|
36. Halawani,
E.M.A. and Shohayeb, M.M. (2011b) Survey of the antibacterial
activity of Saudi and some international honeys. Jpn. Meteorol.
Agency, 3(4): 94-101. |
|
37. Louveaux,
J., Maurizio, A. and Vorwohl, G. (1978) Methods of
melissopalynology. Bee World, 59: 139-157.
https://doi.org/10.1080/0005772X.1978.11097714 |
|
38. Nzeako,
B. and Hamdi, J. (2000) Antimicrobial potential of honey on
some microbial isolates. SQU. J. Sci. Res. Med. Sci., 2:
75-79. |
|
39. Wasfi,
R., Elkhatib, W.F. and Khairalla, A.S. (2016) Effects of
selected Egyptian honeys on the cellular ultrastructure and
the gene expression profile of Escherichia coli. PLoS One,
11(3): e0150984.
https://doi.org/10.1371/journal.pone.0150984 |
|
40.
Cruickshank, R., Duguid, J.P., Masion, B.P. and Swain, R.H.
(1979) Medical Microbiology. 12th ed. Churchill Livingstone,
Edinburgh, London, New York. |
|
41. Zar, J.H.
(1999) Biostatistical Analysis. Prentice Hall, New Jersey,
USA. p663. |
|
42. Feás,
X., Iglesias, A., Rodrigues, S. and Estevinho, L.M. (2013)
Effect of Erica sp. Honey against microorganisms of clinical
importance: Study of the factors underlying this biological
activity. Molecules, 18(4): 4233-4246.
https://doi.org/10.3390/molecules18044233
PMid:23579991 |
|
43.
Sherlock, O., Dolan, A., Athman, R., Power, A., Gethin, G.,
Cowman, S. and Humphreys, H. (2010) Comparison of the
antimicrobial activity of Ulmo honey from Chile and Manuka
honey against methicillin-resistant Staphylococcus aureus,
Escherichia coli and Pseudomonas aeruginosa. BMC Complement.
Altern. Med., 10: 47.
https://doi.org/10.1186/1472-6882-10-47
PMid:20813024 PMCid:PMC2942791 |
|
44. Roth,
L.A., Kwan, S. and Sporns, P. (1986) Use of a disc assay
system to detect oxytetracycline residues in honey. J. Food
Prot., 49(6): 436-441.
https://doi.org/10.4315/0362-028X-49.6.436 |
|
45. Nassar,
H.M., Li, M. and Gregory, R.L. (2012) Effect of honey on
Streptococcus mutans growth and biofilm formation. Appl.
Environ. Microbiol., 78(2): 536-540.
https://doi.org/10.1128/AEM.05538-11
PMid:22038612 PMCid:PMC3255743 |
|
46. Sufya,
N., Matar, N., Kaddura, R. and Zorgani, A. (2014) Evaluation
of bactericidal activity of Hannon honey on slowly growing
bacteria in the chemostat. Drug Healthc. Patient Saf., 6:
139-144.
PMid:25342919 PMCid:PMC4206207 |
|
47. Zainol,
M.I., Mohd Yusoff, K. and Mohd Yusof, M.Y. (2013)
Antibacterial activity of selected Malaysian honey. BMC
Complement. Altern. Med., 13: 129.
https://doi.org/10.1186/1472-6882-13-129
PMid:23758747 PMCid:PMC3716576 |
|
48. Erejuwa,
O.O., Sulaiman, S.A. and Wahab, M.S.A. (2012) Honey - A novel
antidiabetic agent. Int. J. Biol. Sci., 8(6): 913-934.
https://doi.org/10.7150/ijbs.3697
PMid:22811614 PMCid:PMC3399220 |
|
49. Lu, J.,
Carter, D.A., Turnbull, L., Rosendale, D., Hedderley, D.,
Stephens, J., Gannabathula, S., Steinhorn, G., Schlothauer,
R.C., Whitchurch, C.B. and Harry, E.J. (2013) The effect of
New Zealand Kanuka, Manuka and clover honeys on bacterial
growth dynamics and cellular morphology varies according to
the species. PLoS One, 8(2): e55898.
https://doi.org/10.1371/journal.pone.0055898 |
|
50. Mullai,
V. and Menon, T. (2007) Bactericidal activity of different
types of honey against clinical and environmental isolates of
Pseudomonas aeruginosa. J. Altern. Complement. Med., 13(4):
439-442.
https://doi.org/10.1089/acm.2007.6366
PMid:17532737 |
|