Prevalence and antimicrobial resistance pattern of Shiga toxigenic Escherichia coli in diarrheic buffalo calves

Vet World Vol.10 July-2017 Article-11

Research Article

Veterinary World, 10(7): 774-778

https://doi.org/10.14202/vetworld.2017.774-778

M. Srivani¹, Y. Narasimha Reddy², K. V. Subramanyam¹, M. Ramakoti Reddy³, and T. Srinivasa Rao⁴

1. Department of Veterinary Microbiology, NTR College of Veterinary Science, Gannavaram, Krishna - 521 101, Andhra Pradesh, India.
2. Department of Veterinary Microbiology, College of Veterinary Science, Rajendranagar, Hyderabad, Telangana, India.
3. Directorate of Poultry Research, Rajendranagar, Hyderabad, Telangana, India.
4. Department of Veterinary Public Health, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India.

Background and Aim: Aim of the study was to investigate the prevalence, virulence gene profiles, and antimicrobial resistance pattern of Shiga toxigenic Escherichia coli (STEC) in diarrheic buffalo calves from Andhra Pradesh and Telangana States.

Materials and Methods: A total of 375 fecal samples from diarrheic buffalo calves of 1-7, 8-30, 31-60, and 61-90 days age were collected from which STEC were isolated, and virulence genes were detected using multiplex polymerase chain reaction. The antimicrobial resistance of isolates was tested by disk diffusion method.

Results: The prevalence of E. coli associated diarrhea in buffalo calves was 85.04%, of which 35.01% was STEC origin. In STEC, the combination of eaeA and, hlyA virulence genes was highest (42.45%) followed by stx1 (16.04%), stx1, stx2 and hlyA (13.21%), stx2 (12.64%), stx1, eae and hlyA (9.43%) and stx1 and hlyA (6.6%) genes were detected. Highest antimicrobial resistance was observed for tetracycline (63.21%) and ampicillin (48.11%), while chloramphenicol, gentamycin (96.33%) and imipenem (99.06%) antibiotics are susceptible. Multidrug resistance was detected in 69.81% of the STEC isolates from diarrheic buffalo calves.

Conclusion: Higher prevalence of eaeA and hlyA genes carrying isolates of STEC may be a serious zoonotic threat and increased prevalence of multidrug resistance in E. coli may necessitate stringent selection of appropriate antimicrobial agent in treating buffalo calf diarrhea cases. Keywords: antimicrobial resistance, buffalo calf diarrheia, Shiga toxigenic Escherichia coli, virulence genes.

Keywords: antimicrobial resistance, buffalo calf diarrheia, Shiga toxigenic Escherichia coli, virulence genes.

How to cite this article: Srivani M, Reddy YN, Subramanyam KV, Reddy MR, Srinivasa Rao T (2017) Prevalence and antimicrobial resistance pattern of Shiga toxigenic Escherichia coli in diarrheic buffalo calves, Veterinary World, 10(7): 774-778.

Received: 31-10-2016 Accepted: 20-04-2017 Published online: 13-07-2017

Corresponding author: M. Srivani E-mail: srivanimoturi@yahoo.com

DOI: 10.14202/vetworld.2017.774-778

Copyright: Srivani, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.