Open Access
Research
(Published
online: 05-03-2017)
3.
In vitro
evaluation of total mixed ration supplemented with exogenous
fibrolytic enzymes for crossbred cows -
Pravin Mohan Lunagariya,
Ram Sharan Gupta
and Subhash Parnerkar
Veterinary World, 10(3): 281-285
doi:
10.14202/vetworld.2017.281-285
Pravin Mohan
Lunagariya :
Livestock
Research Station, College of Veterinary Science and Animal
Husbandry, Anand Agricultural University, Anand, Gujarat, India.
Ram Sharan Gupta :
Department of
Animal Nutrition Research, College of veterinary Science and
Animal Husbandry, Anand Agricultural University, Anand, Gujarat,
India.
Subhash Parnerkar :
Department of
Animal Nutrition Research, College of veterinary Science and
Animal Husbandry, Anand Agricultural University, Anand, Gujarat,
India.
Received: 18-10-2016, Accepted: 31-01-2017, Published online:
05-03-2017
Corresponding author:
Pravin Mohan
Lunagariya, e-mail: drpravinml@gmail.com
Citation:
Lunagariya PM, Gupta RS, Parnerkar S (2017) In vitro
evaluation of total mixed ration supplemented with exogenous
fibrolytic enzymes for crossbred cows, Veterinary World,
10(3): 281-285.
Abstract
Aim:
The study was
conducted to evaluate the levels of exogenous fibrolytic enzymes (EFE)
on in vitro digestibilities of dry matter (DM) and organic
matter (OM), total gas production (TGP), metabolizable energy (ME)
content, and microbial biomass production (MBP).
Materials and
Methods:
The total mixed
ration (TMR) was prepared using 30% each of sorghum hay and
groundnut straw and 40% compound concentrate mixture to meet
nutritional requirement of cow (500 kg) producing 12 kg fat
corrected milk. The EFE was incorporated at 0, 40, 60, 80, 100,
120, 140, 160, 180, 200, 220, 240, 260, 280, 300, 320, 340, 360,
380, and 400 mg/kg TMR. The TMR substrates with different levels
of EFE were in vitro incubated to ascertain their effect on
digestibility, gas production, and nutritive values.
Results:
The
significantly (p<0.05) higher and optimum in vitro
digestibilities of DM (63.03%) and OM (63.62%) as well as TGP
(72.35 ml/500 mg TMR) were observed at supplementation of 240 mg
EFE/kg TMR, while ME (7.16 MJ/kg DM) and MBP (97.63 mg/500 mg TMR)
were also better.
Conclusion:
The
incorporation of EFE at 240 mg/kg TMR resulted significantly
(p<0.05) higher and optimum in vitro digestibilities of DM
and OM. The TGP, ME, and MBP were also better. The levels of EFE
240 mg/kg TMR were found suitable for further in vivo study
in crossbred cows.
Keywords:
exogenous fibrolytic enzymes, in vitro digestibility,
metabolizable energy, microbial biomass production, total gas
production.
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