Open Access
Research
(Published
online: 06-03-2017)
4.
Prevalence of Salmonella in
poultry processing environments in wet markets in Penang and
Perlis, Malaysia -
Hafiz Nidaullah,
Nadarajan Abirami,
Ahamed Kamal Shamila-Syuhada,
Li-Oon Chuah,
Huda Nurul,
Teik Pei Tan,
Farah Wahida Zainal Abidin
and Gulam Rusul
Veterinary World, 10(3): 286-292
doi:
10.14202/vetworld.2017.286-292
Hafiz Nidaullah :
Food Technology
Division, School of Industrial Technology, Universiti Sains
Malaysia, 11800 Minden, Penang, Malaysia.
Nadarajan Abirami :
Food Technology
Division, School of Industrial Technology, Universiti Sains
Malaysia, 11800 Minden, Penang, Malaysia.
Ahamed Kamal
Shamila-Syuhada :
Food Technology
Division, School of Industrial Technology, Universiti Sains
Malaysia, 11800 Minden, Penang, Malaysia.
Li-Oon Chuah :
Food Technology
Division, School of Industrial Technology, Universiti Sains
Malaysia, 11800 Minden, Penang, Malaysia.
Huda Nurul :
Food Technology
Division, School of Industrial Technology, Universiti Sains
Malaysia, 11800 Minden, Penang, Malaysia.
Teik Pei Tan :
Public Health
Laboratory, Ministry of Health, Jalan Jelapang, 30020 Ipoh, Perak,
Malaysia.
Farah Wahida Zainal
Abidin :
Public Health
Laboratory, Ministry of Health, Jalan Jelapang, 30020 Ipoh, Perak,
Malaysia.
Gulam Rusul:
Food
Technology Division, School of Industrial Technology, Universiti
Sains Malaysia, 11800 Minden, Penang, Malaysia.
Received: 11-09-2016, Accepted: 20-01-2017, Published online:
06-03-2017
Corresponding author:
Gulam Rusul,
e-mail: rusulg@gmail.com
Citation:
Nidaullah H, Abirami N, Shamila-Syuhada AK, Chuah LO, Nurul H, Tan
TP, Zainal Abidin FW, Rusul G (2017) Prevalence of Salmonella
in poultry processing environments in wet markets in Penang
and Perlis, Malaysia, Veterinary World, 10(3): 286-292.
Abstract
Aim:
The aim of
this study was to determine the prevalence of various
Salmonella serotypes in chickens, carcass contact surfaces as
well as environmental samples collected from wet markets and small
scale processing plant.
Materials and
Methods:
A total of 182
poultry and environmental samples were collected at random on
separate occasions from wet markets and small scale processing
plant, during the period of October 2014 to July 2015 in Penang
and Perlis, Malaysia. The samples were analyzed for the presence
of Salmonella using ISO 6579:2002 conventional
culture-based method. Presumptive Salmonella colonies were
subjected to various biochemical tests (such as triple sugar iron
and lysine iron test), serologically confirmed using polyvalent O
and H antisera and further serotyped at Public Health Laboratory,
Ministry of Health, Perak, Malaysia.
Results:
Salmonella
serotypes were isolated from 161 out of 182 samples (88.46%) with
100% prevalence in the whole chicken carcass and chicken cuts - as
well as transport crate, cage, drum, knife, chopping board,
display table, floor, bench wash water, wash water, and drain
water. Salmonella was isolated from 91.67%, 83.33%, and
66.67% of defeathering machines, drain swabs, and apron,
respectively. 17 serotypes were isolated in this study with
Salmonella Albany (57/161), Salmonella Corvallis
(42/161), and Salmonella Brancaster (37/161) being the
predominant serovars.
Conclusion:
The
most carcass contact and environmental samples collected along the
wet market chicken processing line were consistently contaminated
with Salmonella. This indicates that Salmonella has
established itself in poultry processing environments by
colonizing the surfaces of the equipment and survives in these
environments by establishing biofilms. Our results highlight the
need of implementing strict hygiene and sanitation standards to
reduce the incidence of Salmonella. The prevalence of
Salmonella in poultry can be reduced effectively by
identifying and eliminating the sources and contamination sites
during slaughter and processing of poultry.
Keywords:
prevalence, poultry, Salmonella, wet markets.
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