Aim: Aim of the study was to evaluate the effect of isoflurane anesthesia on physiological parameters, assessment of anesthetic qualities, and economy of use of isoflurane in domestic chickens (Gallus domesticus).

Materials and Methods: In this study, 18 apparently healthy adult domestic chickens were selected randomly and divided into three groups. The birds were anesthetized by masked induction with isoflurane at a dose rate of 3.5%, 4%, and 5% and were maintained with 1.5%, 2%, and 2.5% isoflurane with oxygen by endotracheal intubation in Groups I, II, and III, respectively. Physiological parameters, viz., cloacal temperature, heart rate, and respiration rate were recorded at 0, 5, 10, 20, 30, 40, 50, and 60 min. The quality of anesthesia was assessed on the basis of induction time, induction behavior, quality of sedation, production of analgesia, degree of muscle relaxation, palpebral reflex, recovery time, and recovery behavior. The economy of anesthesia was calculated in terms of quantity of isoflurane utilized during 60 min of study. Statistical analysis was performed by analysis of variance, Duncan's multiple range tests.

Results: There was significant decrease (p<0.01) in physiological parameters such as in cloacal temperature, heart rate and respiration rate in the birds of all the groups from 0 to 60 min. The induction time was 5.83±0.33, 2.37±0.18, and 0.87±0.15 min, respectively, in Groups I, II, and III. Induction behavior was smooth in Group III, whereas mildly stormy in Group II and I. Quality of sedation was excellent in Group III, better in Group II as compared to Group I. Analgesia was moderate in Group III whereas poor in Group II and I. Degree of muscle relaxation was excellent in Group III, whereas good in Group I and II. Palpebral reflexes were absent in all the groups. Recovery time was 15.33±0.84, 18.83±0.94, and 26.50±0.85 in Groups I, II, and III respectively. Recovery behavior was smooth in birds of all the groups. The cost of the anesthesia was 158.22±1.04, 194.27±0.66, and 236.84±s0.60 Indian National Rupee in Groups I, II, and III, respectively. Quantity of anesthesia utilized in each group was 7.62±0.05, 9.35±0.03, and 11.41±0.03 ml in Groups I, II, and III, respectively.

Conclusion: The use of isoflurane at different concentration produces different level of physiological changes, quality of anesthesia and economy without causing any deleterious effect on the birds. The physiological parameters observed in this study can serve as reference values for the wild and endangered birds.

Keywords: anesthetic quality, chicken (Gallus domesticus), economy, isoflurane, physiological parameters.

1. Granone, T.D., De Francisco, O.N., Killos, K.B., Quandant, J.E., Mandsager, R.E. and Graham, L.F. (2012) Comparison of three different inhalant anaesthetic agents (Isoflurane, sevoflurane, desflurane) in red-tailed hawks (Buteo jamaicensis). Vet. Anaesth. Anal., 39(1): 29-37. [Crossref] [PubMed]

2. Chistensen, J., Fosse, R.T. and Halvorsen, O.J. (1987) Comparison of various anesthetic regimens in the domestic fowl. Am. J. Vet. Res., 48: 1649-57.

3. Chemonge, S. (2014) Effect of intermittent positive pressure ventilation (IPPV) on depth of anaesthesia during and after isoflurane anaesthesia in sulphur crested cockatoos (Cacatua galerita galerita). Vet. Med. Int., 2014: e250523.

4. Chemonge, S. (2012) Effect of intermittent positive pressure ventilation (IPPV) on acid base balance and plasma electrolytes during isoflurane anaesthesia in sulphur -crested cockatoos (Cacatua galerita galerita). Int. J. Anim. Vet. Adv., 4(6): 351-357.

5. Escobar, A., Thiesen, R., Vitaliano, S.N., Belmonte, E.A., Werther, K. and Valadao, C.A. (2011) Cardiorespiratory effects of isoflurane anaesthesia in crested caracaras (Caracara plancus). J. Zoo Wildl. Med., 42(1): 12-17. [Crossref] [PubMed]

6. Curro, T.G. (1998) Anaesthesia of pet birds: Review article. Semin. Avian Exotic Pet Med., 7(1): 10-21. [Crossref]

7. Jaensch, S.M., Cullen, L. and Radial, S.R. (2001) Comparison of endotracheal, caudal thoracic air sac and clavicular air sac administration of isoflurane in sulphur crested cockatoos (Cacatua galerita). J. Avian Med. Surg., 15(3): 170-177. [Crossref]

8. Ludders, J.W., Mitchell, G.S. and Rode, J. (1990) Minimal anesthetic concentration and cardiopulmonary dose response of isoflurance in ducks. Vet. Surg., 19: 304-307. [Crossref] [PubMed]

9. Sasada, K., Azumano, A., Omiya, T., Watanabe, M., Suzuki, Y. and Suzuki, K. (2014) Improvement of clinical anesthetic techniques in Japanese wild birds. Jpn. J. Vet. Anesth. Surg., 45(2): 19-26. [Crossref]

10. Kilic, N. and Pasa, S. (2009) Cardiopulmonary effects of propofol compared with those of a medetomidine-ketamine combination in the common buzzards (Buteo buteo). Rev. Med. Vet., 160(3): 154-159.

11. Haskins, S.C. (1996) Monitoring the anaesthetized patient. In: Thurmon, J., Tranquilli, J.W. and Benson, C.G., editors. Lumb and Jones Veterinary Anesthesia. Williams & Wilkins, Philadelphia, PA, USA. p409-424.

12. Edling, T.M. (2003) Advances in anesthesia monitoring in birds, reptiles and small mammals. Exotic DVM, 5(3): 15-20.

13. Jaensch, S.M., Cullen, L. and Raidal, S.R. (2002) Air sac functional anatomy of the sulphur-crested cockatoo (Cacatua galerita) during isoflurane anesthesia. J. Avian Med. Surg., 16(1): 2-9. [Crossref]

14. Langlois, I., Harvey, R.C., Jones, M.P. and Schumacher, J. (2003) Cardiopulmonary and anesthetic effects of isoflurane and propofol in hispaniolan amazon parrots (Amazona ventralis). J. Avian Med. Surg., 17(1): 4-10. [Crossref]

15. Mercado, J.A., Larsen, R.S., Wack, R.F. and Pypendop, B.H. (2008) Minimum anaesthetic concentration of isoflurane in captive thick-billed parrots (Rhynchopsitta pachyrhyncha). Am. J. Vet. Res., 69(2): 189-194. [Crossref] [PubMed]

16. Chan, F.T., Chang, G.R., Wang, H.C. and Hsu, T.H. (2013) Anesthesia with isoflurane and sevoflurane in the crested serpent eagle (Spilornis cheela hoya): Minimum anesthetic concentration, physiological effects, hematocrit, plasma chemistry and behavioral effects. J. Vet. Med. Sci., 75(12): 1591-1600. [Crossref] [PMC]

17. English, M.J., Papenberg, R., Farias, E., Scott, W.A.C. and Hinchey, J. (1991) Heat loss in an animal experimental model. J. Trauma., 31: 36-38. [Crossref] [PubMed]

18. Carregaro, A.B.C., Fernando, S.F., Gerardi, P.M. and Scholten, C. (2008) Thermal maintenance in chickens under inhalant anesthesia with isoflurane. Cienc. Rural, 38(5): 1319-1324. [Crossref]

19. Botman, J., Dugdale, A., Gabriel, F. and Vandeweerd, J.M. (2016) Cardiorespiratory parameters in the awake pigeon and during anaesthesia with isoflurane. Vet. Anaesth. Anal., 43: 63-71. [Crossref] [PubMed]

20. Joyner, P.H., Jones, M.P., Ward, D., Gompf, R.E., Zagaya, N. and Sleeman, J.M. (2008) Induction and recovery characteristics and cardiopulmonary effects of sevoflurane and isoflurane in bald eagles. Am. J. Vet. Res., 69: 13-22. [Crossref] [PubMed]

21. Greenless, K.J., Clutton, R.E., Larsen, C.T. and Eyre, P. (1990) Effect of halothane, isoflurane and pentobarbitone anaesthesia on myocardial irritability in chickens. Am. J. Vet. Res., 51(5): 757-758.

22. Escobar, A., Da Rocha, R.W., Pypendop, B.H., Zangirolami, F.D., Sousa, S.S. and Valadao, C.A.A. (2016) Effects of methadone on the minimum anesthetic concentration of isoflurane, and its effects on heart rate, blood pressure and ventilation during isoflurane anesthesia in hens (Gallus gallus domesticus). PLoS One, 11(3): e0152546. [Crossref]

23. Al-Sobayil, F.A., Ahmed, F.A., Al-Wabel, N.A., Al-Thonayian, A.A., Al-Rogibah, F.A., Al-Fuaim, A.H., Al-Obaid, A.O. and Al-Muzaini, A.M. (2009) The use of xylazine, ketamine, and isoflurane for induction and maintenance of anaesthesia in ostriches (Struthio camelus). J. Avian Med. Surg., 23(2): 101-107. [Crossref] [PubMed]

24. Martin-Jurado, O., Simova-Curd, S., Bettschart-Wolfensberger, R. and Hatt, J. (2011) Bispectral index reveals death-feigning behavior in a red kite (Milvus milvus). J. Avian Med. Surg., 25(2): 132-135. [Crossref] [PubMed]

25. Kim, Y.K., Lee, S.S., Suh, E.H., Lee, L., Lee, H.C., Lee, H.J. and Yeon, S.C. (2011) Minimum anesthetic concentration and cardiovascular dose-response relationship of isoflurane in cinereous vultures (Aegypius monachus). J. Zoo Wildl. Med., 42: 499-503. [Crossref] [PubMed]

26. Tomi, K., Mashimo, T., Tashiro, C., Yagi, M., Pak, M., Nishimura, M. and Yoshiya, I. (1993) Alterations in pain threshold and psychomotor response associated with subanaesthetic concentrations of inhalation anaesthetics in humans. Br. J. Anaesth., 70: 684-686. [Crossref] [PubMed]