Genotyping of virulent Escherichia coli obtained from poultry and poultry farm workers using enterobacterial repetitive intergenic consensus-polymerase chain reaction

Vet World Vol.10 November-2017 Article-1

Research Article

Veterinary World, 10(11): 1292-1296

https://doi.org/10.14202/vetworld.2017.1292-1296

M. Soma Sekhar¹, N. Mohammad Sharif², T. Srinivasa Rao¹, and M. Metta³

1. Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Sri Venkateswara Veterinary University, Gannavaram, Andhra Pradesh, India.
2. Department of Veterinary Microbiology, College of Veterinary Science, Tirupati, Andhra Pradesh, India.
3. Department of Animal Genetics and Breeding, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India.

Background and Aim: The aim of this study was to characterize virulent Escherichia coli isolated from different poultry species and poultry farm workers using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) genotyping.

Materials and Methods: Fecal swabs from different poultry species (n=150) and poultry farm workers (n=15) were analyzed for E. coli and screened for virulence genes (stx1, stx2, eaeA, and hlyA) by multiplex PCR. Virulent E. coli was serotyped based on their "O" antigen and then genotyped using ERIC-PCR.

Results: A total of 134 E. coli isolates (122/150 from poultry and 12/15 from farm workers) were recovered. Virulence genes were detected in a total of 12 isolates. Serological typing of the 12 virulent E. coli revealed nine different serotypes (O2, O49, O60, O63, O83, O101, O120, UT, and Rough). ERIC-PCR genotyping allowed discrimination of 12 virulent E. coli isolates into 11 ERIC-PCR genotypes. The numerical index of discrimination was 0.999.

Conclusion: Our findings provide information about the wide genetic diversity and discrimination of virulent E. coli in apparently healthy poultry and poultry farm workers of Andhra Pradesh (India) based on their genotype. Keywords: Escherichia coli, enterobacterial repetitive intergenic consensus-polymerase chain reaction, genotype, poultry, serotype, virulent.

Keywords: Escherichia coli, enterobacterial repetitive intergenic consensus-polymerase chain reaction, genotype, poultry, serotype, virulent.

How to cite this article: Sekhar MS, Sharif NM, Rao TS, Metta M (2017) Genotyping of virulent Escherichia coli obtained from poultry and poultry farm workers using enterobacterial repetitive intergenic consensus-polymerase chain reaction, Veterinary World, 10(11): 1292-1296.

Received: 22-07-2017 Accepted: 03-10-2017 Published online: 01-11-2017

Corresponding author: M. Soma Sekhar E-mail: somasekharmadupuru@gmail.com

DOI: 10.14202/vetworld.2017.1292-1296

Copyright: Sekhar, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.