Aim: Assessment of the status of subclinical mastitis (SCM) in Jamunapari and Barbari goats in Indian organized farms, the involvement of bacterial pathogens and their sensitivity to antibiotics.

Materials and Methods: A total of 181 composite milk samples were aseptically collected from the apparently healthy Barbari (n=95) and Jamunapari (n=86) goats. The California mastitis test (CMT) and somatic cell count (SCC) were used to diagnose SCM. The milk samples with CMT scores of 0 and +1 were considered as negative, while the samples with the score of +2 or +3 were taken as positive, and further, the positive samples were used for the bacteriological examination. An antibiotic sensitivity test was performed by disk diffusion method using seven commercially available antibiotic discs.

Results: All the samples having CMT score of +2 or +3 demonstrated SCC more than 1 million. Overall, the prevalence of SCM in the goats was assessed as 19.89% (36/181). The prevalence of SCM in Barbari and Jamunapari goats was found as 24.21% (23/95) and 15.12% (13/86), respectively. Out of 11 isolates of Staphylococci, 9 isolates were identified as coagulase-negative Staphylococci (CNS), whereas 2 isolates were found as Staphylococcus aureus. The identified bacterial isolates (n=30) did not show antibiotic resistance.

Conclusion: The current investigation showed the considerable prevalence of SCM among Jamunapari and Barbari goats which may have a negative impact on quantity and quality of the milk. CNS was found as the most prevalent cause of SCM in the goats. Negligible antibiotic resistance was found among the identified udder pathogens.

Keywords: California mastitis test, coagulase-negative Staphylococci, goat, somatic cell count, subclinical mastitis.

1. Sharma, N., Mukherjee, R. and Mishra, A.K. (2013) In: Covali, A. and Jian, A., editors. Preventive and Therapeutic Modalities for Control of Bovine Mastitis. Lambert Academic Publishing, Saarbrucken.

2. Sharma, N., Mukherjee, R., Ingale, S.L. and Jadhav, R.K. (2010) Effect of Phyllanthus emblica on ceruloplasmin in bovine staphylococcal mastitis. Indian J. Vet. Res., 19(2): 19-24.

3. Thompson-Crispi, K., Atalla, H., Miglior, F. and Mallard, B.A. (2014) Bovine mastitis: Frontiers in immunogenetics. Front. Immunol., 5: 1-10. [Crossref] [PubMed] [PMC]

4. Mishra, A.K., Shalini, Sharma, N., Kumar, A. and Shivasharanappa, N. (2014) Prevalence of subclinical mastitis in different breeds of goats. Vet. Pract., 15(1): 140-141.

5. Ali, Z., Muhammad, G., Ahmad, T., Khan, R., Naz, S., Anwar, H. and Usama, A.R. (2010) Prevalence of caprine sub-clinical mastitis, its etiological agents and their sensitivity to antibiotics in indigenous breeds of Kohat, Pakistan. Pak. J. Life Soc. Sci., 8: 63-67.

6. Persson, Y. and Olofsson, I. (2011) Direct and indirect measurement of somatic cell count as indicator of intramammary infection in dairy goats. Acta. Vet. Scand., 53(15): 1-5. [Crossref]

7. Islam, A., Samad, A. and Rahman, A.K.M.A. (2012) Prevalence of subclinical caprine mastitis in Bangladesh based on parallel interpretation of three screening tests. Int. J. Anim. Vet. Adv., 4: 225-228.

8. Virdis, S., Scarano, C., Cossu, F., Spanu, V., Spanu, C. and de Santis, E.P.L. (2010) Antibiotic resistance in Staphylococcus aureus and coagulase negative Staphylococci isolated from goats with subclinical mastitis. Vet. Med. Int., 2010: 517060. [Crossref] [PubMed] [PMC]

9. Padhy, A., Sahu, A.R., Shekhar, S., Sahoo, S., Sahoo, A. and Dalai, N. (2015) Staphylococcus aureus: An emergent cause of bovine mastitis in India-a review. Int. J. Livest. Res., 5(2): 1-7. [Crossref]

10. Mishra, A.K., Sharma, N., Kumar, A., Kumar, N., Gundallahalli, B.M.R., Kumar, S. and Kumar, N. (2014) Isolation, characterization and therapeutic potential assessment of bacteriophages virulent to Staphylococcus aureus associated with goat mastitis. Iran. J. Vet. Res., 15(4): 320-325. [PubMed] [PMC]

11. Graibawi, M.A., Sharma, V.K. and Al-Shammari, A.J. (1986) Microbial pathogens from goat mastitis and phage-typing of Staphylococcus aureus isolates. Comp. Immun. Microbiol. Infect. Dis., 9(1): 23-28. [Crossref]

12. Bergonier, D., Cremoux, R.D., Rupp, R., Lagriffoul, G. and Berthelot, X. (2003) Mastitis of dairy small ruminants. Vet. Res., 34: 689-716. [Crossref] [PubMed]

13. Sharma, N., Singh, N.K. and Bhadwal, M.S. (2011) Relationship of somatic cell count and mastitis: An overview. Asian Aust. J. Anim. Sci., 24(3): 429-438. [PubMed]

14. Leon-Galvan, M.F., Barboza-Corona, J.E., Lechuga-Arana, A.A., Valencia-Posadas, M., Aguayo, D.D., Cedillo-Pelaez, C. and Gutierrez-Chavez, A.J. (2015) Molecular detection and sensitivity to antibiotics and bacteriocins of pathogens isolated from bovine mastitis in family dairy herds of central Mexico. Biomed. Res. Int., 2015: 615153. [Crossref]

15. Hogan, J.S., Gonzales, R.N. and Harmon, R.J. (2009) Laboratory Handbook on Bovine Mastitis. National Mastitis Council, Madison, Wisconsin, USA.

16. Gebrewahid, T.T., Abera, B.H. and Menghistu, H.T. (2012) Prevalence and etiology of subclinical mastitis in small ruminants of Tigray Regional state, North Ethiopia. Vet. World, 5: 103-109. [Crossref]

17. Schlam, O.W., Carrol, E.J. and Jain, N.C. (1971) Bovine Mastitis. Lea and Febiger, Philadelphia, PA. p128-129.

18. Shearer, J.K. and Harris, B.Jr. (2003) Mastitis in Dairy Goats. Animal Science University of Florida, Gainesville, USA.

19. Mbindyo, C.M., Gitao, C.G. and Bebor, L. (2014) A cross-sectional study on the prevalence of subclinical mastitis and antimicrobial susceptibility patterns of the bacterial isolates in milk samples of smallholder dairy goats in Kenya. Am. J. Res. Commun., 2(8): 30-51.

20. Cowan, S.T. and Steel K.J. (1975) Manual for Identification of Medical Bacteria. Cambridge University Press, London, U.K.

21. Edwards, R. and Ewing, W.N. (1986) Identification of Enterobacteriaceae. Elsevier Publishing, New York, U.S.A.

22. Kreig, N.R. and Holt, J.G. (1984) Burgeys Manual of Systematic Bacteriology. Springer Publishing, Williams and Wilkins, New York, U.S.A.

23. Quinn, P.J., Markey, B.K., Leonard, F.C., Hartigan, P., Fanning, S. and Fitzpatrick, E.S. (2011) Veterinary Microbiology and Microbial Disease. Wiley-Blackwell, Hoboken, New Jersey, U.S.A.

24. National Committee of Clinical Laboratory Standards. (2006) Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, Approved Standard M7-A7, Eleventh Informational Supplement, NCCLS Document M 100-911. Clinical and Laboratory Standards Institute, NCCLS, Wayne, PA, Pensylvania, USA.

25. Bourabah, A., Ayad, A., Boukraa, L., Hammoudi, S.M. and Benbarek, H. (2013) Prevalence and etiology of subclinical mastitis in goats of the Tiaret region, Algeria. Glob. Vet., 11(5): 604-608.

26. Contreras, A., Sierra, D., Sanchez, A., Corrales, J.C., Marcoc, J.C., Paape, M.J. and Gonzalo, C. (2007) Mastitis in small ruminants. Small Rum. Res., 68: 145-153. [Crossref]

27. Haftay, A., Habtamu, T.M. and Abebe, M.S. (2016) Bacterial identification and antimicrobial susceptibility of subclinical mastitis causing bacteria from goats in Aba'lla district, Afar, North-Eastern Ethiopia. Rev. Med. Vet., 167(7-8): 170-175.

28. Islam, M.A., Samad, M.A. and Rahman, A.A. (2011) Bacterial pathogens and risk factors associated with mastitis in black Bengal goats in Bangladesh. Bangl. J. Vet. Med., 9(2): 155-159.

29. Sharma, N., Mukherjee, R. and Mishra, A.K. (2013) Assessment of therapeutic potential of autogenous Staphylococcus aureus bacterin plus Vitamin E and selenium in mouse model mastitis. Indian. Vet. J., 90(5): 38-39.

30. Brahma, B., Dutta T.K., De, S., Banik, S., Bhakat, M. and Roy, B. (2011) Mastitis pathogens from crossbred cows in North Western Himalayas. Indian. Vet. J., 88(10): 155-159.

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