Aim: The aim of this study was to determine the effect of a wound dressing consisting of a zinc oxide with turmeric extract combination as an anti-inflammatory on the healing process through the expression of MAC387 and cyclooxygenase-2 (COX-2).

Materials and Methods: Forty Wistar rats were divided into four control and four treatment groups (n=5). On day 1, a 6 mmx6 mm square of skin in the area of the vertebralis thoracis was excised from all subjects. The wound was then dressed with a combination of zinc oxide and turmeric extract for the treatment groups, while the control groups were left undressed. Both the control and treatment groups were then sequentially sacrificed on days 3, 5, 7, and 14 to obtain subepithelial excision samples. These samples subsequently underwent immunohistochemistry examination through the expression of MAC387 and COX-2 to ascertain the anti-inflammatory reaction to the wound healing process.

Results: The highest expression of MAC387 was found in the treatment group to which a dressing of zinc oxide with turmeric extract had been applied on the day 5 before slowly reducing on days 7 and 14. MAC387 peaked in the undressed control group on day 14. The COX-2 expression results in control groups showed their higher expression on day 3, increased up to day 5, began to decline on day 7 before, and finally, decreasing on day 14. This result was different to those treatment groups which presented a high COX-2 expression on day 3, before gradually decreasing between days 5 and 7 and reaching its lowest point on day 14.

Conclusion: A wound dressing consisting of a combination of zinc oxide and turmeric extract has been proven effective as an anti-inflammatory in the healing process.

Keywords: cyclooxygenase-2, MAC387, turmeric, wound dressing, zinc oxide.

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