Metabolic and morphometric changes in Indonesian cynomolgus monkeys (Macaca fascicularis) fed an atherogenic diet composed of locally sourced ingredients

Research (Published online: 22-11-2018)

14. Metabolic and morphometric changes in Indonesian cynomolgus monkeys (Macaca fascicularis) fed an atherogenic diet composed of locally sourced ingredients

Sri Rahmatul Laila, Dewi Apri Astuti, Irma Herawati Suparto, Ekowati Handharyani and Dondin Sajuthi

Veterinary World, 11(11): 1609-1617

Sri Rahmatul Laila: Department of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.

Dewi Apri Astuti: Primate Research Center, Bogor Agricultural University, Bogor, Indonesia; Department of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, Bogor, Indonesia.

Irma Herawati Suparto: Primate Research Center, Bogor Agricultural University, Bogor, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Indonesia.

Ekowati Handharyani: Primate Research Center, Bogor Agricultural University, Bogor, Indonesia Department of Veterinary Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.

Dondin Sajuthi: Primate Research Center, Bogor Agricultural University, Bogor, Indonesia Department of Veterinary Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.

doi: 10.14202/vetworld.2018.1609-1617

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Article history: Received: 29-06-2018, Accepted: 10-10-2018, Published online: 22-11-2018

Corresponding author: Sri Rahmatul Laila

E-mail: sajuthi@indo.net.id

Citation: Laila SR, Astuti DA, Suparto IH, Handharyani E, Sajuthi D (2018) Metabolic and morphometric changes in Indonesian cynomolgus monkeys (Macaca fascicularis) fed an atherogenic diet composed of locally sourced ingredients, Veterinary World, 11(11): 1609-1617.

Abstract

Background and Aim: This study was designed to determine the effects of a new atherogenic diet formulated at Institut Pertanian Bogor (IPB) (Bogor, Indonesia) on metabolic, morphometric, and carotid artery imaging of cynomolgus monkeys.

Materials and Methods: A total of 20 adult male cynomolgus monkeys fed IPB-1 atherogenic diet for 1 year. Total plasma cholesterol (TPC), high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, triglycerides, and morphometric measurements were evaluated at baseline and monthly during the study. Carotid plaques and intima-media thickness (IMT) were measured using ultrasonography at baseline and after 8 months of treatment.

Results: This diet increased TPC, LDL, and TPC/HDL ratio and induced carotid atherosclerosis in this model. The TPC, LDL, and TPC/HDL ratio were positively associated; however, HDL was negatively associated with carotid plaques and IMT.

Conclusion: The IPB-1 atherogenic diet formulated with locally and readily available ingredients provides an economically and scientifically feasible monkey model to study atherosclerosis in Indonesia and Southeast Asia.

Keywords: atherogenic diet, carotid artery, cynomolgus, ultrasonography.

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