Serological detection of avian reovirus in different poultry flocks of Gazipur and Mymensingh districts of Bangladesh

Research (Published online: 26-07-2019)

30. Serological detection of avian reovirus in different poultry flocks of Gazipur and Mymensingh districts of Bangladesh

Syeda Farjana Neepa, Zobayda Farzana Haque, Abdullah Al Momen Sabuj, Md Alimul Islam and Sukumar Saha

Veterinary World, 12(7): 1126-1131

Syeda Farjana Neepa: Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Zobayda Farzana Haque: Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abdullah Al Momen Sabuj: Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Md Alimul Islam: Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Sukumar Saha: Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

doi: 10.14202/vetworld.2019.1126-1131

Share this article on [Facebook] [LinkedIn]

Article history: Received: 26-12-2018, Accepted: 28-05-2019, Published online: 26-07-2019

Corresponding author: Sukumar Saha

E-mail: sukumar.saha@bau.edu.bd

Citation: Neepa SF, Haque ZF, Sabuj AA, Islam MA, Saha S (2019) Serological detection of avian reovirus in different poultry flocks of Gazipur and Mymensingh districts of Bangladesh, Veterinary World, 12(7): 1126-1131.

Abstract

Background and Aim: Avian reovirus (ARV) is a constraint to poultry industry in Bangladesh as a cause of several diseases in chickens, especially in broiler. However, the actual status of the viral infection is not known because the large-scale study is not conducted in this country. Therefore, this study aimed to check the presence and distribution of ARV-specific antibody in respect to area, types of chickens (broiler breeder, broiler, and layer), vaccination status, and age of chickens in Gazipur and Mymensingh districts of Bangladesh.

Materials and Methods: A total of 276 chickens' blood samples were collected from two well-organized broiler breeder stock, seven broiler farms, and five layer farms located at two districts, namely Gazipur and Mymensingh of Bangladesh. Blood samples were collected from wing vein of the apparently healthy chickens using 3 ml of syringe and serum was harvested by keeping the syringe at room temperature in slanting position. The sera were transferred to the laboratory by maintaining the cool chain and further processing was performed by indirect enzyme-linked immunosorbent assay using ARV antibody test kit.

Results: The results of serological test revealed that an average of 39.5% seropositive against ARV was recorded in chickens of Gazipur and Mymensingh districts. Among these, chickens of Gazipur district had the highest seropositivity of 50.5% than Mymensingh (30.7%). With respect to vaccination status, the seropositivity of vaccinated chickens in both areas was 100% and non-vaccinated chickens was 50.5% in Gazipur and 30.7% in Mymensingh district, respectively. However, regarding age groups, the seropositivity was higher in the age of 4-6 weeks (64.5%).

Conclusion: The present serological findings showed a higher prevalence of ARV-specific antibodies in broiler birds. It indicates that the poultry industries of Bangladesh are contaminated with ARV which may naturally be transmitted to chickens either vertically or horizontally.

Keywords: avian reovirus, enzyme-linked immunosorbent assay, seropositive.