Vet World Vol.12 March-2019 Article-17
Research Article
Veterinary World, 12(3): 459-465
https://doi.org/10.14202/vetworld.2019.459-465
Influence of some parameters on the ability of Listeria monocytogenes, Listeria innocua, and Escherichia coli to form biofilms
2. Biology Department, Faculty of Sciences of Nature and Life and Earth Sciences, University of Bouira, Algeria.
Background and Aim: The present study was conducted to evaluate the capacity of Listeria monocytogenes (L.m), Listeria innocua (L.i), and Escherichia coli to form biofilms on polystyrene support under different parameters by performing crystal violet (CV) staining technique.
Materials and Methods: Different suspensions were prepared with single strains and with multiple combinations of strains including two serogroups of L.m (IIa and IIb), L.i, and E. coli strains at different microbial load. Selected strains and combinations were grown in biofilms for 6 days attached to polystyrene microplates under aerobic and microaerophilic conditions. The evaluation of the power of adhesion and biofilm formation was determined by CV staining followed by the measurement of optical density at 24 h, 72 h, and 6 days incubation time with and without renewal of the culture medium.
Results: All the strains tested, presented more or less adhesion power depending on the variation of the studied parameters as well as the ability to form multispecies biofilms. Their development is more important by renewing the culture medium and increasing the initial load of bacteria. The ability to adhere and form biofilms differs from one serogroup to another within the same species. In bacterial combination, strains and species of bacteria adopt different behaviors.
Conclusion: The ability to form biofilms is a key factor in the persistence of tested strains in the environment. Our study showed that L.m, L.i, and E. coli could adhere to polystyrene and form biofilms under different conditions. More researches are necessary to understand the mechanisms of biofilm formation and the influence of different parameters in their development. Keywords: biofilm, Escherichia coli, Listeria innocua, Listeria monocytogenes, polystyrene support, variation of parameters.
Keywords: biofilm, Escherichia coli, Listeria innocua, Listeria monocytogenes, polystyrene support, variation of parameters.
How to cite this article: Lezzoum-Atek S, Bouayad L, Hamdi TM (2019) Influence of some parameters on the ability of Listeria monocytogenes, Listeria innocua, and Escherichia coli to form biofilms, Veterinary World, 12(3): 459-465.
Received: 28-11-2018 Accepted: 11-02-2019 Published online: 26-03-2019
Corresponding author: Sara Lezzoum-Atek E-mail: sara_lezzoum@hotmail.fr
DOI: 10.14202/vetworld.2019.459-465
Copyright: Lezzoum-Atek, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.