Bacillus subtilis and lactic acid bacteria improve the growth performance and blood parameters and reduce Salmonella infection in broilers

Research (Published online: 16-12-2020)

12. Bacillus subtilis and lactic acid bacteria improve the growth performance and blood parameters and reduce Salmonella infection in broilers

Nalisa Khochamit, Surasak Siripornadulsil, Peerapol Sukon and Wilailak Siripornadulsil

Veterinary World, 13(12): 2663-2672

Nalisa Khochamit: Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.

Surasak Siripornadulsil: Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand; Research Center for Environmental and Hazardous Substance Management, Khon Kaen University, Khon Kaen 40002, Thailand; Center of Excellence on Hazardous Substance Management (HSM), Patumwan, Bangkok, 10330 Thailand.

Peerapol Sukon: Research Group for Preventive Technology in Livestock, Department of Anatomy, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.

Wilailak Siripornadulsil: Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand; Research Center for Environmental and Hazardous Substance Management, Khon Kaen University, Khon Kaen 40002, Thailand; Center of Excellence on Hazardous Substance Management (HSM), Patumwan, Bangkok, 10330 Thailand.

doi: www.doi.org/10.14202/vetworld.2020.2663-2672

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Article history: Received: 09-07-2020, Accepted: 02-11-2020, Published online: 16-12-2020

Corresponding author: Wilailak Siripornadulsil

E-mail: swilai@kku.ac.th

Citation: Khochamit N, Siripornadulsil S, Sukon P, Siripornadulsil W (2020) Bacillus subtilis and lactic acid bacteria improve the growth performance and blood parameters and reduce Salmonella infection in broilers, Veterinary World, 13(12): 2663-2672.

Abstract

Aim: The aim of the study was to determine the potentials and effects of Bacillus subtilis and lactic acid bacteria (LAB) as probiotics on broiler growth, health, and Salmonella infection.

Materials and Methods: To evaluate the inoculum size applicable for broilers, 1-day-old broilers were orally fed fresh cultures of single strains and a B. subtilis KKU213/Pediococcus pentosaceus NP6 mixture at 10⁸ and 10¹² colony-forming unit (CFUs)/mL/chick. The body weight gain (BWG), Salmonella contamination level and total Bacillus and LAB abundances in the crop and intestine were measured. Subsequently, 1-day-old broilers were orally fed of KKU213, CH403, and Pediococcus acidilactici SH8 at 10¹⁰ CFUs/mL, followed by inulin. After 35 days, the BWG, Bacillus and LAB abundances in the cecum, blood parameters, and KKU213 colonization were assessed.

Results: The broilers fed single strains or KKU213+NP6 exhibited a higher BWG and a higher crop LAB abundance than the controls (p<0.05). Probiotic feeding decreased the intestinal Salmonella abundance and correspondingly increased the LAB abundance. The broilers fed the mixed culture (KKU213+CH403+SH8) followed by prebiotics showed lower mortality, higher blood high-density lipoprotein levels, and lower blood uric acid levels than the controls (p<0.0004). Probiotic feeding significantly increased the Bacillus and LAB counts (p<0.05). A CE330 isolate obtained from the cecum after 35 days of KKU213 feeding was closely related to B. subtilis KKU213.

Conclusion: B. subtilis KKU213 is a potent probiotic strain that can survive, colonize and reduce Salmonella infection in broilers and improve their growth and health. This strain, combined with different LAB can act synergistically in the gut and promote broiler growth.

Keywords: broiler, Bacillus subtilis, lactic acid bacteria, probiotics.