Anti-feline immunodeficiency virus reverse transcriptase properties of some medicinal and edible mushrooms

Research (Published online: 05-09-2020)

8. Anti-feline immunodeficiency virus reverse transcriptase properties of some medicinal and edible mushrooms

Supaphorn Seetaha, Siriluk Ratanabunyong, Lueacha Tabtimmai, Kiattawee Choowongkomon, Jatuporn Rattanasrisomporn and Khuanjarat Choengpanya

Veterinary World, 13(9): 1798-1806

Supaphorn Seetaha: Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand; Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Siriluk Ratanabunyong: Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand; Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Lueacha Tabtimmai: Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Kiattawee Choowongkomon: Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Jatuporn Rattanasrisomporn: Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand; Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.

Khuanjarat Choengpanya: Program in Applied Biology, Maejo University Phrae Campus, Phrae 54140, Thailand.

doi: www.doi.org/10.14202/vetworld.2020.1798-1806

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Article history: Received: 19-03-2020, Accepted: 06-07-2020, Published online: 05-09-2020

Corresponding author: Jatuporn Rattanasrisomporn

E-mail: fvetjpn@ku.ac.th

Citation: Seetaha S, Ratanabunyong S, Tabtimmai L, Choowongkomon K, Rattanasrisomporn J, Choengpanya K (2020) Anti-feline immunodeficiency virus reverse transcriptase properties of some medicinal and edible mushrooms, Veterinary World, 13(9): 1798-1806.

Abstract

Background and Aim: Feline immunodeficiency virus (FIV) causes AIDS-like symptoms in domestic and wild cats. Treatment of infected cats has been performed using human anti-HIV drugs, which showed some limitations. This study aimed to determine the anti-FIV potential of some mushrooms.

Materials and Methods: A total of 17 medicinal and edible mushrooms were screened to find their inhibitory effect against FIV reverse transcriptase (FIV-RT). Three solvents, water, ethanol, and hexane, were used to prepare crude mushroom extracts. Fluorescence spectroscopy was used to perform relative inhibition and 50% inhibitory concentrations (IC₅₀) studies.

Results: The ethanol extract from dried fruiting bodies of Inonotus obliquus showed the strongest inhibition with an IC₅₀ value of 0.80±0.16 μg/mL. The hexane extract from dried mycelium of I. obliquus and ethanol and water extracts from fresh fruit bodies of Phellinus igniarius also exhibited strong activities with the IC₅₀ values of 1.22±0.20, 4.33±0.39, and 6.24±1.42 μg/mL, respectively. The ethanol extract from fresh fruiting bodies of Cordyceps sinensis, hexane extracts from dried mycelium of I. obliquus, ethanol extracts of Ganoderma lucidum, hexane extracts of fresh fruiting bodies of Morchella esculenta, and fresh fruiting bodies of C. sinensis showed moderate anti-FIV-RT activities with IC₅₀ values of 29.73±12.39, 49.97±11.86, 65.37±14.14, 77.59±8.31, and 81.41±17.10 μg/mL, respectively. These mushroom extracts show anti-FIV potential.

Conclusion: The extracts from I. obliquus, P. igniarius, C. sinensis, and M. esculenta showed potential anti-FIV activity.

Keywords: feline immunodeficiency virus, fluorescence spectroscopy, mushrooms, crude extracts, reverse transcriptase.