Hematology and toll-like receptors 2 and 4 and lipopolysaccharide-induced tumor necrosis factor-α factor gene expression in peripheral blood mononuclear cells of Thai indigenous chickens

Vet World Vol.15 December-2022 Article-5

Research Article

Veterinary World, 15(12): 2795-2799

https://doi.org/10.14202/vetworld.2022.2795-2799

Chananphat Tantikositruj¹, Asep Gunawan², Muhammad Jasim Uddin³, Wirawan Nuchchanart⁴, Chaiwat Boonkaewwan⁵, Watchara Laenoi⁶, and Autchara Kayan¹

1. Department of Animal Science, Faculty of Agriculture, Kasetsart University, Ngam Wong Wan, Bangkok 10900, Thailand.
2. Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor 16680, Indonesia.
3. School of Veterinary Medicine, Murdoch University, Western Australia 6155, Australia.
4. Department of Animal Science, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
5. Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80161, Thailand.
6. Department of Animal Science, School of Agriculture and Natural Resources, University of Phayao, Phayao 56000, Thailand.

Background and Aim: Toll-like receptors (TLRs) play crucial roles in the early phase of infection in the innate immune response against bacteria, viruses, fungi, and parasites. Lipopolysaccharide-induced tumor necrosis factor-α factor (LITAF) is an essential transcription factor that regulates the immune system, apoptosis, and inflammatory cytokines. This study aimed to determine the hematological profile reflecting the immune response related to TLR2 and TLR4 and LITAF gene expression in Thai indigenous chickens.

Materials and Methods: Blood samples (2 mL) were randomly obtained from three chicken breeds (black-boned chicken, Fah Luang chicken, and Pradu Hang Dam chicken) at 16 weeks of age (n = 5 per breed). The hematological profile and mRNA expression within the peripheral blood mononuclear cells (PBMCs) were determined by hematological analysis and quantitative real-time polymerase chain reaction (qRT-PCR).

Results: The hematological profile differed significantly in terms of red blood cells (RBCs), hemoglobin, and white blood cells (WBCs) (p < 0.05). Black-boned chicken and Fah Luang chicken had lower RBC levels than Pradu Hang Dam chicken. Fah Luang chicken had lower hemoglobin than Pradu Hang Dam chicken. However, Fah Luang chicken had higher WBC levels than Pradu Hang Dam chicken. Hematocrit, heterophils, basophils, eosinophils, lymphocytes, and monocytes did not differ significantly among the groups (p > 0.05). According to qRT-PCR, the expression of the TLR2 gene did not differ significantly among the groups (p > 0.05), while TLR4 and LITAF gene expression did (p < 0.05). Toll-like receptor 4 and LITAF genes were most highly expressed in Fah Luang chicken.

Conclusion: The PBMCs of Thai indigenous chickens showed evidence of TLR4 and LITAF gene expression, with higher expression levels observed in Fah Luang chicken. From this preliminary study, it is concluded that TLR4 and LITAF genes might play roles in the main immune system response in Thai indigenous chickens. Keywords: blood hematology, gene expression, immune, Thai indigenous chicken.

Keywords: blood hematology, gene expression, immune, Thai indigenous chicken.

How to cite this article: Tantikositruj C, Gunawan A, Uddin MJ, Nuchchanart W, Boonkaewwan C, Laenoi W, and Kayan A (2022) Hematology and toll-like receptors 2 and 4 and lipopolysaccharide-induced tumor necrosis factor-α factor gene expression in peripheral blood mononuclear cells of Thai indigenous chickens, Veterinary World, 15(12): 2795–2799.

Received: 30-06-2022 Accepted: 26-10-2022 Published online: 07-12-2022

Corresponding author: Autchara Kayan E-mail: fagrark@ku.ac.th

DOI: 10.14202/vetworld.2022.2795-2799

Copyright: Tantikositruj, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.