Antimicrobial susceptibility test and antimicrobial resistance gene detection of extracellular enzyme bacteria isolated from tilapia (Oreochromis niloticus) for probiotic candidates

Research (Published online: 11-02-2023)

4. Antimicrobial susceptibility test and antimicrobial resistance gene detection of extracellular enzyme bacteria isolated from tilapia (Oreochromis niloticus) for probiotic candidates

Mira Mawardi, Agustin Indrawati, I. Wayan Teguh Wibawan, and Angela Mariana Lusiastuti

Veterinary World, 16(2): 264-271

Mira Mawardi: School of Veterinary Medicine and Biomedical Sciences (SVMBS), IPB University, Jl. Agatis Kampus IPB Dramaga Bogor, 16680, Indonesia; Main Center for Freshwater Aquaculture, Ministry of Marine Affairs and Fisheries, Jl. Selabintana No. 37, Selabatu, Kec, Cikole, Kota Sukabumi, Jawa Barat 43114, Indonesia.

Agustin Indrawati: School of Veterinary Medicine and Biomedical Sciences (SVMBS), IPB University, Jl. Agatis Kampus IPB Dramaga Bogor, 16680, Indonesia.

I. Wayan Teguh Wibawan: School of Veterinary Medicine and Biomedical Sciences (SVMBS), IPB University, Jl. Agatis Kampus IPB Dramaga Bogor, 16680, Indonesia.

Angela Mariana Lusiastuti: Research Center for Veterinary Sciences, National Research and Innovation Agency, RE Martadinata 30 Bogor, Jawa Barat, Indonesia.

doi: www.doi.org/10.14202/vetworld.2023.264-271

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Article history: Received: 22-10-2022, Accepted: 13-01-2023, Published online: 11-02-2023

Corresponding author: Agustin Indrawati

E-mail: indraseta@apps.ipb.ac.id

Citation: Mawardi M, Indrawati A, Wibawan IWT, and Lusiastuti AM (2023) Antimicrobial susceptibility test and antimicrobial resistance gene detection of extracellular enzyme bacteria isolated from tilapia (Oreochromis niloticus) for probiotic candidates, Veterinary World, 16(2): 264–271.

Abstract

Background and Aim: Antimicrobial resistance (AMR) is a global problem that can increase mortality and morbidity rates and adversely affect health. Therefore, AMR control must be carried out in various sectors, including the fisheries sector, using probiotics. Bacteria can become resistant to antibiotics, including bacteria used for probiotics. This study aimed to isolate bacteria as potential producers of extracellular enzymes, phenotypic characterization, and antibiotic-resistant gene patterns.

Materials and Methods: In this study, 459 bacterial isolates were isolated from the stomach of tilapia in Indonesia. Tilapia was obtained from Sukabumi, Ciamis, Serang, Banjarnegara, Jayapura, Sorong, Manokwari Selatan, Takalar, Lampung, Batam, and Mandiangin. Enzymatic bacteria were identified. An antimicrobial susceptibility test was conducted by agar disk diffusion, and genotypic detection of encoding genes was performed using a molecular method.

Results: This study obtained 137 isolates (29.84%) that can produce extracellular enzymes. The highest number of E-sensitive isolates was found, including 130 isolates (94.89%). Six isolates (6/137) can produce four enzymes (amylase, protease, cellulose, and lipase), and they were sensitive to antibiotics. A total of 99 isolates can produce extracellular enzymes, and they were sensitive to antibiotics. Such isolates serve as a consortium of probiotic candidates. The isolates that are resistant to oxytetracycline (OT), erythromycin (E), tetracycline (TE), and enrofloxacin (ENR) included 15 isolates (10.95%), seven isolates (5.11%), three isolates (2.19%), and one isolate (0.73%), respectively. In addition, four isolates (2.92%) were detected as multidrug-resistant. The tet(A) gene obtained the highest result of detection of resistance genes in isolates that were intermediate and resistant to TE and OT. Isolates that serve as ENR intermediates have a high qnr(S) resistance gene.

Conclusion: The data in this study provide the latest update that bacteria can serve as a consortium of potential probiotics with antibiotic-resistant genes for the treatment of fish. Bacteria that are intermediate to antibiotics may contain resistance genes. The results of this study will improve the policy of probiotic standards in Indonesia.

Keywords: antibiotic, antimicrobial resistance, enzymatic bacteria, Oreochromis niloticus, probiotic.