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Review
11.
Diagnosis and molecular characterization of chicken anaemia
virus -
Nishant Singh Saini and Anjan Dandapat
Vet World. 2009; 2(4): 156-160
Abstract
Chicken infectious anemia (CIA) is an emerging disease
especially of young chickens and has proved considerable health
problems and economic losses to the poultry industry worldwide.
The disease is characterized by aplastic anemia, hemorrhages in
the muscle and subcutaneous tissue, thymus atrophy and
immunosuppression. CIA infection is relatively easy to identify
based on the pathognomic signs and lesions exhibited by the
affected flock.Tentatively it can usually be made based on flock
history, clinical signs, haematological changes and gross
pathological findings in affected birds. For confirmatory
diagnosis isolation and identification of the CIAV is done.
Reduced haematocrit (PCV) values are the sensitive indicator to
identify clinically affected birds with CIAV following
experimental exposure. Monitoring of CIAV infection by virus
isolation, antigen and CIAV-specific antibody detection by
enzyme linked immunosorbent assay (ELISA), virus neutralization
test (VNT), immunofluorescent test (IFT) and immunoperoxidase
test (IPT), along with application of molecular diagnostic tools
such as polymerase chain reaction (PCR), nucleic acid
hybridization and sequencing etc. can be used for confirmatory
diagnosis of CIAV infection.
Keywords:
Chicken, Diagnosis, Virus, Molecular characterisation, Economic,
Industry
