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Research
1.
Natural occurrences of ochratoxicosis
in slaughtered pigs from different regions of Serbia -
Dragan Milicevic, Verica Juric, Dubravka Vukovic, Miodrag Mandic
Veterinary World 2(8): 293-298
Abstract
Samples of
blood, kidney and liver per animal were randomly selected from
slaughtered pigs (n=60) and analyzed for ochratoxin A.
Determination of ochratoxin A (OTA) concentration in samples of
kidney and liver was performed by HPTLC after immunoaffnity
column clean-up, while for plasma samples, spectrofluorometric
procedure was used. Of the 60 plasma samples, 60% contained OTA
in the range of 2.5-33.3 ng/mL (mean 3.05±5.0 ng/mL), while the
incidence of OTA in kidneys and liver were very similar (70% and
65%). The average OTA concentration in liver was 3.2±4.35 ng/g
(1.2-19.5 ng/g) and in kidneys was 3.97±4.47 ng/g (1.3-22.0 ng/g).
A statistically significant difference (p<0.01) was found
between region B. Topola and Kovilj for both liver and kidney
samples. Also, in kidney originating from region Kovilj and
Senta a statistically significant difference (p<0.01) was found.
Mean distribution followed the pattern:kidney>liver>serum
(100>80.8>77%). The results from these survey indicated that
there was a strong correlation between the OTA level in serum
and liver as well as in the OTA serum in kidney (r=0.884 and
r=0.896, respectively) while the strongest correlation was found
between the OTA level in liver and in kidney (r=0.970).
Keywords:
Ochratoxin A, Tissue, Residue, Swine, Slaughter.
