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Research
3.
Comparison of structural polypeptides of two field isolates of
IBH virus
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S. K. Saxena, R. Chandra, R Kumar and D. Kumar
Veterinary World 2(8): 301-303
Abstract
Inclusion
Body Hepatitis (IBH) caused by adenovirus is a reemerging
diseaseof mainly young broiler chicks of
3-5 weeks age. The polypeptide pattern of a local isolate of
virus causing Inclusion Body Hepatitis was analyzed by sodium
dodecyl slulphate-polyacrylamide gel electrophoresis. A total of
9 polypeptides ranging in molecular weight between 35.2 and
110.0 kDa were observed. Western blot analysis of structural
polypeptides also revealed that all 9 polypeptides were
immunogenic. Out of these 9 polypeptides, 2 viz 97.4 kDa and
53.9 kDa were found to be major immunogenic polypeptide based on
intense staining.
Keywords : Inclusion
Body Hepatitis, Immunogenic polypeptides, Structural
polypeptides.
