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Research
1.
Prevalence of Peste Des Petits Ruminants Virus (PPRV) in Mardan,
Hangu and Kohat district of Pakistan; comparative analysis of
PPRV suspected serum samples using competitive ELISA (cELISA)
and agar gel immunodiffusion (AGID) -
Aslam Misbah, Muhammad Abubakar, Rehana Anjum, Shamim Saleha,
Qurban Ali
Vet World. 2009; 2(3): 89-92
doi: 10.5455/vetworld.2009.89-92
Abstract
Peste des
petits Ruminants (PPR) is an acute, febrile, highly contagious
and economically important viral disease of small ruminants.
However PPR is more prevalent in sheep and goat. Competitive
ELISA, Virus neutrilization test, and RT-PCR are the
available techniques for diagnosis of PPR, which give rapid
detection where as Agar gel immunodiffusion and Counter
immunoelectrophoresis were previously used for PPR
detection. In this study two serological techniques were
compared for PPR diagnosis. The main aim of this study was to
evaluate the comparative sensitivity of both techniques for PPR
detection. For this purpose one hundred and sixty PPR
suspected serum samples collected from goats and sheep flocks
(unvaccinated) from three Districts of NWFP including Mardan,
Hangu and Kohat were analyzed in National Veterinary
Laboratories, Islamabad. Out of these 160 samples, fifty (50)
were found positive for PPR antibodies with cELISA (Prevalence =
31.25%). The cELISA positive serum samples however gave negative
results when tested with AGID although the control well was
always positive. Thus it was concluded that cELISA
technique is more sensitive and specific than AGID for PPR
antibody detection.
Keywords:
Prevalence,
PPR, cELISA and AGID
