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Research
3.
Evaluation of group specific nested PCR for detection of
bluetongue virus -
H. C. Chauhan, H. N. Kher, B. S. Chandel, A. I. Dadawala, L.
Jain, S. M. Agrawal and A. Bhadaniya
Vet World. 2009; 2(5): 179-182
Abstract
During the
present study RT-PCR and nested PCR was evaluated for the
detection of BTV in blood samples collected from suspected cases
of bluetongue. Due to conserved nature, NS1 gene was targeted
for the development of partial length and nested PCR assay. The
partial length RT-PCR and nested PCR assays yielded a specific
PCR product of expected 274 bp and 101 bp sizes, respectively.
In, the present study out of 68 blood samples processed for BTV
detection, 2 samples were found positive for BTV genome by
RT-PCR as well as in nested PCR. The described BTV PCR based
assay provides a valuable tool to study the epidemiology of BTV
infection in susceptible domestic livestock.
Keywords:
Ruminant,
Mortality, Genome, PCR assays, Epidemiology, Economic.
