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Research
3.
Comparison of rose bengal plate agglutination, standard tube
agglutination and indirect ELISA tests for detection of brucella
antibodies in cows and buffaloes -
S. N. Ghodasara, Ashish Roy and B. B. Bhanderi
Vet World. 2010; 3(2): 61-64
Abstract
A total of 180 serum samples (107 cows, 73 buffaloes) from cases
of abortion and various reproductive disorders were collected
for detection of Brucella antibody by Rose Bengal Plate
Agglutination Test (RBPT), Serum Tube Agglutination Test (STAT)
and indirect- ELISA (i-ELISA). The overall prevalence of
brucellosis by RBPT, STAT and i-ELISA were 11.21%, 16.00% and
24.30% in cows 9.59%, 12.33% and 26.03% in buffaloes
respectively. Overall seroprevalence of Brucellosis in cases of
abortion, R.O.P. by RBPT, STAT and i-ELISA were 11.32%, 16.04%
and 32.08% respectively. When three serological tests were
compared, seropositivity was found highest by i-ELISA (25%),
followed by STAT (14.45%) and RBPT (10.56%). The results shows
higher prevalence of brucellosis in cases of abortion and
retention of placenta, while at lower level from various
reproductive disorders as detected serologically indicating
endemicity of the infection in villages around Anand city,
Gujarat.
Keywords: Brucella, Gynaecological disorders, Cows,
Buffaloes.
