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Research
2.
Seed germination inhibition test for pregnancy
detection in Malnad Gidda cows -
M. Narayana Swamy, C. Ravikumar and G. P. Kalmath
Vet World. 2010; 3(3): 107-108
Abstract
In the present study, the seed germination
inhibition technique was applied to diagnose
pregnancy in Malnad Gidda cattle breed, a dwarf
breed found in coastal and neighboring heavy
rainfall areas in Karnataka. The urine samples
collected from six inseminated Malnad Gidda cows at
two months of post insemination served as positive
group and the urine from six non inseminated Malnad
Gidda cows served as negative group. In both the
cases, the urine was diluted at the ratio of 1:4
with distilled water. In each sterile Petri dish
fifteen wheat seeds were taken on the blotting paper
and 15 ml of diluted urine was added. For each cow
the test was conducted with a replica of six tests
in six Petri dishes. Control test was also carried
out with the addition of water only to the wheat
seeds. The Petri dishes were covered with the trays
to avoid evaporation. After three days, the seeds
were examined for germination inhibition percentage
in positive, negative and control groups, wherein
the mean germination inhibition percentage was 73.65
± 2.81, 27.90 ± 2.56 and 21.48 ± 2.69, respectively.
The mean shoot length of the germinated wheat seeds
on fifth day was 0.95 ± 0.47, 3.62 ± 0.51 and 5.54 ±
0.68 cm in positive, negative and control groups,
respectively. Mean germination inhibition percent
and reduced shoot length in positive group of Malnad
Gidda cattle was indicative of pregnancy state. It
was concluded that the seed germination inhibition
technique is useful to detect pregnancy in Malnad
Gidda cattle as a simple, non-invasive and
economical method.
Keywords: Pregnancy Diagnosis, Cow, Seed
Germination, Urine.
