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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Review Article
11.
Temporal expression of marker transcripts: Key to
successful maturation and development of mammalian
oocytes -
A Nath, V Sharma, N E Gade, M D Pratheesh, R kumar
and
P K Dubey
Vet World. 2012; 5(2): 121-127
doi:
10.5455/vetworld.201 2.121-127
Abstract
In vitro embryo production (IVEP) is one of the
tool to gain rapid genetic improvement in
livestock besides its use in conservation of
endangered species. IVEP provides necessary
starting material for the basic research in animal
development, embryo genomics and stem cells. The
success of IVEP depends upon several major factors
such as quality of oocyte and sperms, type of
culture medium, culture conditions etc. In vitro
produced embryos differ substantially from in vivo
derived embryos in the mRNA expression patterns of
genes important for development. As female
germplasm (oocyte) is more valuable when compared
to male germplasm (sperm) because of scanty
availability and relatively fixed number, it is
essential to maintain quality of oocyte in IVEP.
Oocyte maturation is an inevitable step towards
successful IVEP as the process of maturation is
still not clearly revealed, it is essential to
identify temporal expression pattern of marker
genes involved during maturation process for
production of developmentally competent embryos.
Mammalian models can provide better insight into
the oocyte maturation mechanism. This review
focuses on the various marker genes which are
essential for successful maturation of mammalian
oocyte and are key regulators in deciding the fate
of oocytes for production of developmentally
competent embryos.
Keywords: Oocytes,
Maturation, Embryo, Developmental competence,
Marker transcripts
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