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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Original Research
2.
Influence of addition of different antibiotics in
semen diluent on viable bacterial count and
spermatozoal viability of Awassi ram semen -
O I Azawi and M A Ismaeel
Vet World. 2012; 5(2): 75-79
doi:
10.5455/vetworld.201 2.75-79
Abstract
The objectives of the present study were to
determine the effects of six different antibiotics
in controlling the growth of semen contaminating
bacteria and if these antibiotics have any adverse
effect on Awassi ram spermatozoa. Semen samples
from six mature Awassi rams were used in this
study. A total number of 120 ejaculates were
collected from the rams using an artificial vagina
once a week. Semen ejaculates were evaluated for
volume, sperm concentration, mass motility,
individual motility, percentage live sperm, sperm
abnormalities, and viable bacterial count. Semen
samples were diluted by sodium
citrate-fructose-egg yolk. The diluted semen
sample was divided into 7 parts. Six types of
antibiotics were added to the semen diluent parts
including; penicillin G 1000 IU ml-1 with
streptomycin 1 mg ml-1, gentamicin sulphate 250 mg
ml-1, tetracycline 0.5 mg ml-1, lincomycin 1 mg
ml-1, cefoperazone sodium 1mg ml-1, cefdinir 1 mg
ml-1 and the seventh part considered as a control
group without antibiotic addition. The diluted
semen samples were cooled and preserved at 5 Co
for 5 days. Cooled diluted semen samples were
examined for individual motility, percent of live
sperm, sperm abnormalities, acrosomal defects and
bacterial count every 24 h until 5 days. Comparing
with the control, all the antibiotics examined
were effective in controlling bacterial growth
(P<0.05) from 24 h to 96 h of preservation at 5
Co. Cefdinir and cefoperazone sodium proved to be
significantly (P<0.05) effective than other
antibiotics in controlling bacterial growth at 96
h of preservation as the bacterial count were 23.3
± 3.7 x 103 / ml and 25.4 ± 6.2 x 103 / ml,
respectively. Lincomycin, gentamicin sulphate and
tetracycline proved ineffective in controlling
bacterial growth at 96 h of preservation as the
bacterial count were 57.1 ± 20.1 x 103 / ml, 52.5
± 29.4 x 103 / ml and 46.5 ± 8.8 x 103 / ml,
respectively. The addition of tetracycline to
diluted ram semen significantly reduced (P<0.05)
sperm individual motility and percent live sperm
and a significant increase (P<0.05) acrosomal
defects was observed at 96 h of preservation in
comparison to control and other antibiotics. Sperm
viability was highly correlated with bacterial
count in the control part of diluted semen (r =
0.794; P < 0.01). It could be concluded from the
results of the present study that additions of
cephalosporins (cefdinir or Cefoperazone sodium)
at the dose of 1 mg ml-1 were most effective
amongst the antibiotics used in checking the
bacterial growth and improving semen quality of
Awassi ram.
Keywords: Awassi ram; Semen;
Dilution; Antibiotics; Bacterial count.
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