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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Original Research
5.
Disinfectant effect of methylated ethanol against
Listeria species -
Y Yakubu, M D Salihu, O O Faleke, M B Abubakar, A
A Magaji and A U Junaidu
Vet World. 2012; 5(2): 91-93
doi:
10.5455/vetworld.201 2.91-93
Abstract
This study was carried out in order to determine
the disinfectant effect of Methylated spiritŪ (95%
methanol and 5% ethanol) as a teat dip against
Listeria species. Hand milking was employed to
collect 576 (288 x 2) raw milk samples from
different lactating cows within Sokoto metropolis
(Nigeria). 288 samples were collected before
disinfecting the udder teats with Methylated
spiritŪ, while the other 288 were collected after
disinfection with Methylated spiritŪ. The samples
were analyzed using selective culture and
isolation technique in which the 288 samples
collected before disinfection, 114 (39.6%) were
positive for Listeria species. Among the positive
samples 44 (38.6%) were Listeria innocua, 16
(14.0%) Listeria ivanovii, 36 (31.6%) Listeria
monocytogenes, 11 (9.6%) Listeria welshimeri and 7
(6.1%) Listeria seeligeri, while none of the 288
samples collected after disinfection was positive.
The study has shown high prevalence of Listeria
species in milk collected without
washing/disinfecting the teats and has also
established the sensitivity of Listeria species to
methylated ethanol which can be used as dip for
disinfecting udder teats before milking in order
to prevent contamination with Listeria species and
other methylated spirit-sensitive organisms. This
study is essential to educate Fulani herdsmen and
other milk handlers on the importance of
disinfecting udder teats before milking.
Keywords: Raw milk, Disinfection, Methylated
ethanol, Listeria monocytogenes, Public health
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