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Research
(Published
online: 26-12-2014)
18. Antioxidative capacity of vitamin E,
vitamin C and their combination in cryopreserved Bhadavari bull
semen -
Pawan Kumar Mittal, Mukul Anand, A. K. Madan,
S. Yadav and J. Kumar
Veterinary World, 7(12): 1127-1131
doi:
10.14202/vetworld.2014.1127-1131
Pawan
Kumar Mittal:
Department of Physiology, College of Veterinary Sciences and
Animal Husbandry, Pt. Deen Dayal Upadhyaya Pashu
Chikitsa
Vishwavidyalaya Evam Go Anusndhan Sansthan, Mathura, Uttar
Pradesh, India;
drpavanmittal111@gmail.com
Mukul
Anand:
Department of Physiology, College of Veterinary Sciences and
Animal Husbandry, Pt. Deen Dayal Upadhyaya Pashu Chikitsa
Vishwavidyalaya Evam Go Anusndhan Sansthan, Mathura, Uttar
Pradesh, India;
drmukulanandvet@gmail.com
A. K.
Madan:
Department of Physiology, College of Veterinary Sciences and
Animal Husbandry, Pt. Deen Dayal Upadhyaya Pashu Chikitsa
Vishwavidyalaya Evam Go Anusndhan Sansthan, Mathura, Uttar
Pradesh, India;
akumarmadan@yahoo.com
S.
Yadav:
Department of Physiology, College of Veterinary Sciences and
Animal Husbandry, Pt. Deen Dayal Upadhyaya Pashu Chikitsa
Vishwavidyalaya Evam Go Anusndhan Sansthan, Mathura, Uttar
Pradesh, India;
yadavsarvajeet24@gmail.com
J.
Kumar: Department of Physiology, College of Veterinary
Sciences and Animal Husbandry, Pt. Deen Dayal Upadhyaya Pashu
Chikitsa Vishwavidyalaya Evam Go Anusndhan Sansthan, Mathura,
Uttar Pradesh, India;
jkvermadr@rediff.com
Received:
18-08-2014, Revised: 10-11-2014, Accepted: 15-11-2014, Published
online: 26-12-2014
Corresponding author:
Mukul Anand, e-mail: drmukulanandvet@gmail.com
Abstract
Aim:
The aim was to determine the antioxidative capacity of vitamin
E, vitamin C and their combination (vitamin E+C) on standard semen
parameters i.e., mortality, percent live sperms, percent abnormal
sperms and acrosomal integrity in cryopreserved Bhadawari bull
semen.
Materials and Methods: Ejaculates collected from four
Bhadawari bulls were evaluated and later pooled at 37°C. Pooled
semen sample was divided into four equal parts viz.
T-1,T-2,T-3 and C. The divided samples were diluted with a
Trisbased extender containing the antioxidants vitamin E (5 mM)
(T-1) vitamin C (5 mM) (T-2), vitamin E+C combination (5
mM+5 mM) (T-3) and extender containing no antioxidants (control,
C). The diluted samples with different antioxidant concentration
were cooled to 5°C, frozen in 0.25-ml French straws and stored in
liquid nitrogen ( −196°C).
Frozen straws were thawed individually at 37°C for 20 s in a water
bath for evaluation.
Results: Semen extender supplementation with vitamin E (5 mM),
vitamin C (5 mM) and vitamin E+C combination caused significant
(p<0.01) increases in the seminal attributes while significant
(p<0.01) decreases was observed in percent abnormal sperms in
comparison to control group indicating that supplementation of
antioxidant in form of vitamin and their combination improves the
quality of post thaw semen. Significantly higher values of semen
parameters were observed in the T-3 followed by T-1 and T-2
indicating the anti-oxidative capacity of different supplements
utilized during the study.
Conclusion: The results of present investigation concluded
that combination of vitamin E+C has most profound effect in
protecting sperms against reactive oxygen species production and
cold shock when compared to vitamin E and vitamin C supplemented
alone in the extender for semen dilution utilized for
cryopreservation.
Keywords: antioxidants, Bhadawari bull,
cryopreservation, semen, vitamin E, vitamin C.
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