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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online: 28-02-2014)
11. Isolation, identification, characterization and
antibiogram of Pasteurella multocida isolated from pigs in Mizoram with special
reference to progressive atrophic rhinitis - Zomuankima Varte, T. K.
Dutta, P. Roychoudhury, Jubeda Begum and Rajesh Chandra
Veterinary World, 7(2): 9 5-99
doi:
10.14202/vetworld.2014.95-99
Abstract
Aim: Isolation and
identification of Paturella multocida from pigs of Mizoram with or
without clinical lesions of progressive atrophic rhinitis (PAR)
followed by their detection, capsular typing and screening for
toxA gene.
Materials and Methods: Four hundred nasal swabs sampled
from pigs were collected from 6 different districts of Mizoram.
Swabs were processed for detection and isolataion of P. multocida
by PCR and traditional bacteriological assays. Isolates were
subjected for multiplex PCR for capsular typing, detection of toxA
gene and characterization by RAPD-PCR. Isolates were also tested
for antimicrobial sensitivity profile by disc-diffusion method.
Results: A total of 21 swabs were found to be positive by
P. multocida species specific PCR (PM-PCR) with an amplicon of 460
bp, of which P. multocida could be isolated from 15 swabs (3.75%).
All the isolates were grouped under capsular type A (n=9) and D
(n=6) by multiplex PCR. All the isolates were found to be negative
for toxA gene by PCR, indicating the isolates as non-toxigenic.
Isolates under similar capsular type provided a unique banding
pattern by RAPD-PCR, whereas no discrimination was observed
between serotypes.
Conclusion: Although, no isolates exhibited toxA gene by
PCR, isolataion of P. multocida of capsular type A and D may be an
indication of their possible role in PAR in pigs of Mizoram.
Keywords: Mizoram, PAR, Pasteurella multocida
References
|
1. Rushton, J. (2009) Diseases of pigs. In : The economica of
animal health and production. CAB International, Oxfordshire,
UK. Pp : 248-255. |
|
|
|
2. OIE Terrestrial Manual (2012) Atrophic Rhinitis of Swine.
Chapter 2. 8. 2. |
|
|
|
3. Hamilton, T.D., Roe, J.M. and Webster, A.J.E. (1996)
Synergistic role of gaseous ammonia in etiology of Pasteurella
multocida as well as bacteria free crude extracts and purified
dermonecrotic toxin of Pasteurella multocida. Am. J. Vet. Res.
49: 1844-1849. |
|
|
|
4. Markam, S.K., Khokhar, R.S., Kapoor, S. and Kadian, S.K.
(2009) Molecular characterization of field isolates of
Pasteurella multocida by polymerase chain reaction in the
state of Haryana. Indian J. Comp. Microbiol. Immunol. Infect.
Dis., 30 (1): 31-34. |
|
|
|
5. Babita Devi L (2012) Molecular characterization of
Pasteurella multocida of porcine origin. Ph.D. theis.
Submitted to Assam Agricultural University, Jorhat, Assam. |
|
|
|
6. Dutta, T.K., Singh, V.P. and Kumar, A.A. (2009) Molecular
detection and characterization of Indian isolates of
Pasteurella multocida serogroup D. Indian. J. Anim. Sci. 79:
11-14. |
|
|
|
7. CLSI. (2011) Performance standards for antimicrobial
susceptibility testing, 21st international supplement. CLSI
M100-S21. Clinical and Laboratory Standards Institute, Wayne,
PA. |
|
|
8. Biswas, A., Shivachandra, S.B., Saxena, M.K., Kumar, A.A.,
Singh, V.P. and Srivastava, S.K., (2004) Molecular variability
among strains of Pasteurella multocida isolated from an
outbreak of haemorrhagic septicemia in India. Vet. Res. Comm.,
28: 287-298.
http://dx.doi.org/10.1023/B:VERC.0000026656.77847.5b |
|
|
9. Kalorey, D.R., Yusuraj, S., Vanjari, S.S., Gunjal, P.S.,
Dhanawade, N.B., Barbuddhe, S.B. and Bhandarkar, A.G. (2008)
PCR analysis of Pasteurella multocida isolates from an
outbreak of pasteurellosis in Indian pigs. Comp. Immunol.
Microbiol. Infect. Dis., 31: 495-465.
http://dx.doi.org/10.1016/j.cimid.2007.06.003 |
|
|
10. Amigot, J.A., Torremorell, M. and Pijoan, C. (1998)
Evaluation of techniques for the detection of toxigenic
Pasteurella multocida strains from pigs. J. Vet. Diagn.
Invest., 10: 169-173.
http://dx.doi.org/10.1177/104063879801000209 |
|
|
11. Townsend, K.M., Boyce, J.D., Chung, J.Y., Frost, A.J. and
Adler, B. (2001) Genetic organization of Pasteurella multocida
cap Loci and development of a multiplex capsular PCR typing
system. J. Clin. Microbiol., 39: 924-929.
http://dx.doi.org/10.1128/JCM.39.3.924-929.2001 |
|
|
12. Choi, C., Kim, B., Cho, W.S., Kim, J., Kwon, D., Cheon,
D.S. and Chae, C. (2001) Capsular serotype, toxA gene and
antimicrobial susceptibility profiles of Pasteurella multocida
isolated from pigs with pneumonia in Korea. Vet. Rec.,149:
210–212.
http://dx.doi.org/10.1136/vr.149.7.210 |
|
|
|
13. Zhao, G., Pijoan, C., Murtaugh, M.P. and Molitor, T.W.
(1992) Use of restriction endonuclease analysis and ribotyping
to study epidemiology of Pasteurella multocida in closed swine
herds. Infect. Immunol, 60: 1401–1405. |
|
|
14. Gardner, I.A., Kasten, R., Eamens, G.J., Snipes, K.P. and
Anderson, R.J. (1994) Molecular fingerprinting of Pasteurella
multocida associated with progressive atrophic rhinitis in
swine herds. J. Vet. Diagn. Invest., 6: 442–447.
http://dx.doi.org/10.1177/104063879400600407 |
|
|
|
15. Ranjan, R., Panda, S.K., Acharya, A.P., Singh, A.P. and
Gupta, M.K. (2011) Molecular diagnosis of Haemorrhagic
Septicaemia- A review. Vet. World, 4: 189-192. |
|
|
|
16. Dutta, T.K., Gautam, R., Singh, V.P. and Kumar, A.A.
(2004) Molecular detection and characterization of Indian
isolates of Pasteurella multocida serogroup 'A'. Indian. J.
Anim. Sci., 74: 244-247. |
|
|
|
17. Sharma, S.P., Sharma, R.K. and Aahman, H. (2005)
Isolation, serotyping and antibiogram of Pasteurella from pigs
of North-East India. Indian. J. Anim. Sci., 74: 905-907. |
|
|
18. Portis, E., Lindeman, C., Johansen, L. and Stoltman, G.
(2012) A ten-year (2000-2009) study of antimicrobial
susceptibility of bacteria that cause bovine respiratory
disease complex--Mannheimia haemolytica, Pasteurella multocida,
and Histophilus somni--in the United States and Canada. J Vet
Diagn Invest. 24(5):932-44.
http://dx.doi.org/10.1177/1040638712457559 |
|
|
