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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online: 11-02-2014)
4. Isolation and prevalence of Salmonella from chicken
meat and cattle milk collected from local markets of Patna, India -
Purushottam Kaushik, Anjay, Savita Kumari, Sanjay Kumar Bharti and Shanker Dayal
Veterinary World, 7(2): 62-65
doi:
10.14202/vetworld.2014.62-65
Abstract
Aim: To evaluate the hygienic
quality of raw chicken meat and raw milk sold in the local markets
of Patna with reference to isolation of Salmonella and antibiotic
resistance pattern of Salmonella against commonly used
antibiotics.
Materials and Methods: A total of 370 samples comprising of
228 chicken meat and 142 market milk samples were processed for
isolation and serotyping, supplemented with molecular detection of
isolates targeting invA gene of Salmonella. All the isolates were
tested against commonly used antibiotics (13 nos).
Results: Out of 370 samples, 23.7% (54/228) chicken meat
and 7.7% (11/142) milk samples were found positive for Salmonella
based on biochemical reactions. The serotyping of Salmonella
isolates showed an incidence of 6.1% of Salmonella typhimurium,
2.6% of S. newport, 1.7% of S. gallinarum and 0.4% each of S.
enteritidis, S. infantis and S. worthington in chicken meat; and
2.1% of S. typhimurium and 1.4% of S. newport in market milk
samples. Polymerase chain reaction targeting invA gene showed
positive presence of Salmonella in 18.42% chicken meat and 5.6%
market milk samples.
Conclusion: The antibiotic susceptibility test revealed the
presence of multiple drug resistant Salmonella in chicken meat and
milk. The present study indicates high prevalence of Salmonella in
raw chicken meat and milk due to poor hygienic practices and
therefore emphasizes the need for adopting these hygienic
practices.
Keywords: antibiotic resistance, invA, Salmonella,
serotypes.
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