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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online: 18-02-2014)
8. Biochemical changes following electro-acupuncture
and static magnetic field therapy in rabbits for bone defect healing
- Girraj Shakya, M. M. S. Zama, H. P. Aithal, Reihii John and Mahendra Baghel
Veterinary World, 7(2):
83-86
doi:
10.14202/vetworld.2014.83-86
Abstract
Aim: To evaluate the effect
of Electro-Acupuncture (EA) and Static Magnetic Field (SMF)
therapy on healing of bone defect in rabbits.
Materials and Methods: A 2 mm bone defect was created at
radial midshaft of 18 New Zealand White rabbits. The animals were
then randomly divided into 3 equal groups. The animals in the
group A did not receive any physiotherapy and served as control.
Group B animals were treated with the EA using 50 Hz and 3-10 mA
DD current for 10 min. Group C animals were treated with SMF
therapy on alternate days till day 15. Activity of serum calcium
(Ca), potassium (P) and ALP; Plasma Cortisol and LPO were
evaluated preoperatively and on days 7, 15, 30 and 60 of
experiment.
Results: Postoperatively, SMF therapy treated animals
exhibited higher Ca, P and ALP level and low initial level of
Cortisol and LPO as compared to EA and control group animal.
Conclusion: SMF and EA had positive effect on bone defect
healing and early resolution of stress, induced due to creation of
bone defect, however SMF had an edge over EA.
Keywords: bone defect healing, electro acupuncture, static
magnetic field.
References
1. Steyn, P. E., Ramey, D. W., Kirschvink, J., and Uhrig, J.
(2000) Effect of SMF on blood flow to the metacarpus in
horses. J. Am. Vet. Assoc., 217: 874-877.
http://dx.doi.org/10.2460/javma.2000.217.874 |
|
2. Jansen, J. H., Van der Jagt, O. P., Punt, B. J., Verhaar,
J. A., van Leeuwen, J. P., Weinans, H., and Jahr, H. (2010)
Stimulation of osteogenic differentiation in human
osteoprogenitor cells by pulsed electromagnetic fields: an in
vitro study. BMC Musculoskelet Disord. 23(11):188.
http://dx.doi.org/10.1186/1471-2474-11-188 |
|
3. Bruce, G. K., Howlett, C. R. and Huckstep R. L. (1987)
Effect of a static magnetic field on fracture healing in
rabbit radius: preliminary results. Clin Orthop Relat Res 222:
300–306. |
|
4. Saifzadeh, S., Rezazadeh, G., Naghadeh, B. D. and Ajodani,
M. (2006) Enhancement of bone healing by static magnetic field
in the dog: biomechanical study. Iranian Journal of Veterinary
Surgery, 1: 58-64. |
|
5. Huang, H. M., Lee, S. Y., Yao, W. C., Lin, C. T. and Yeh,
C. Y. (2006) Static magnetic fields up-regulate osteoblast
maturity by affecting local differentiation factors. Clin
Orthop Relat Res 447: 201–208.
http://dx.doi.org/10.1097/01.blo.0000203464.35561.be |
|
6. Morris, C. E. and Skalak T. C. (2008) Acute exposure to a
moderate strength static magnetic field reduces edema
formation in rats. Am. J. Physiol. Heart Circ. Physiol.,
294(1): 50-57.
http://dx.doi.org/10.1152/ajpheart.00529.2007 |
|
7. Null, G. (1998) Healing with magnets. 1st Edn., New York,
carroll and Graf publishers Inc., PP: 120-135. |
|
8. Fu, H. (2000) What is material base of acupuncture ? The
nerves. Medical Hypothesis., 54(3): 358-359.
http://dx.doi.org/10.1054/mehy.1999.0846 |
|
9. Bakhtiari, J. and Zama, M. M. S. (1998) Annual
International Congress on Veterinary Acupuncture, pp: 186-
194. |
|
10. Zama, M. M. S., Gupta, O. P., Singh, G. R. and Swarup, D.
(1999) Post operative acupuncture therapy in fracture of
femur: clinical, haematological and biochemical studies in
goats. Indian J Vet Surg, 20: 86-87. |
|
11. Nakajima, M., Inoue, M., Hojo, T., Inoue, N., Tanaka, K.,
Takatori, R. and Itoi, M. (2010) Effect of EA on the healing
process of tibia fracture in a rat model: a randomised
controlled trial. Acupunct Med. 28: 140-143.
http://dx.doi.org/10.1136/aim.2009.001800 |
|
12. Amarpal., Kinjavdekar, P., Aithal, H. P., Pawde, A. M. and
Pratap, K. (2010) A comparision of xylazine-ketamine,
acepromazine-ketamine, and medetomidine-ketamine combination
for general anaesthesia in rabbits. Scand. J. Lab. Anim. Sci.,
37: 223-229. |
|
13. Asghari, A., Haghdost, I. S. and Gharachorlou, A. (2011)
Evaluation of the effect of different amounts of vitamin D3 in
bone healing and maintenance of serum vitamin D and Ca
concentration in the rabbit. Advances in Environmental
Biology, 5(7): 1548-1553. |
|
14. Rao, T. M., Lakshmipathi, G. V., Sastry, T. P. and
Ramakrishna, O. (2001) Biochemical changes following ulnar
segmental defect repair with fibrillar collagen - hydroxyl-apetite
and porous hydroxy-apetite implant in canines. Indian J. Anim.
Res., 35(2): 112 - 115. |
|
15. Darendililer, M., Darendililer, A. and Sinclair, P. (1997)
Effect of static magnetic and pulsed electromagnetic fields on
bone healing. Int. J. Adult Orth. Orthog. Surg., 12: 45-53. |
|
16. Shen, M., Qi, X., Huang, Y., Lü, Y. and Cai, W. (1999)
Effects of acupuncture on the pituitary-thyroid axis in
rabbits with fracture. J. Tradit. Chin. Med., 19: 300-3. |
|
17. Paskalev, M., Krastev, S. and Filipov, J. (2005) Changes
in some serum bone markers after experimental fracture and
intramedullary osteosynthesis in dogs. Trakia Journal of
Sciences, 3(5): 46-50. |
|
18. Tembhurne, R.D., Gahlod, B.M., Dhakate, M.S., Akhare S.B.,
Upadhye S. V., and Bawaskar S. S. (2010) Management of femoral
fracture with the use of horn peg in canine. Vet. World.,
3(1): 37-41. |
|
19. Yamamoto, Y., Ohsaki, Y., Goto, T., Nakasima, A. and
Iijima, T. (2003) Effects of SMFs on bone formation in rat
osteoblast cultures. J. Dent. Res., 82: 962-66.
http://dx.doi.org/10.1177/154405910308201205 |
|
20. Cheng, R., McKibbin, L., Roy, B. and Pomeranz, B. (1980)
EA elevates blood cortisol levels in naive horses; sham
treatment has no effect. Int. J. Neurosci., 10: 95-97.
http://dx.doi.org/10.3109/00207458009160487 |
|
21. Zare, S., Hayatgeibi, H., Alivandi, S. and Ebadi, A. G.
(2006). Effects of 50hz magnetic field on some factors of the
immune system in male guinea pigs. Internet J Med Tech, 3(1):
1-6. |
|
22. Prasad, G., Dhillon, M. S., Khullar, M. and Nagi, O. N.
(2003). Evaluation of oxidative stress after fractures: A
preliminary study. Acta Orthopædica Belgica, 69(6): 546- 551. |
|
23. Yu, Y. P., Ju, W. P., Li, Z. G., Wang, D. Z., Wang, Y. C.
and Xie, A. M. (2010). Acupuncture inhibits oxidative stress
and rotational behavior in 6- hydroxydopamine lesioned rat.
Brain Res., 1336: 58-65.
http://dx.doi.org/10.1016/j.brainres.2010.04.020 |
|
|