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Research
(Published
online: 03-07-2014)
2. Assessment of humoral immunity to
Eimeria tenella sporozoites in chickens by ELISA - S.
Saravanan, K. M. Palanivel, T. J. Harikrishnan, P. Srinivasan and
G. Selvaraju
Veterinary World, 7(7): 452-456
doi:
10.14202/vetworld.2014.452-456
S. Saravanan: Department of Veterinary Epidemiology and
Preventive Medicine, Veterinary College and Research Institute,
Tamil Nadu Veterinary and Animal Sciences University, Namakkal,
Tamil Naud, India; sarvet_25@yahoo.com
K. M. Palanivel: Department of Veterinary Epidemiology and
Preventive Medicine, Veterinary College and Research Institute,
Tamil Nadu Veterinary and Animal Sciences University, Namakkal,
Tamil Naud, India; drkmpalanivel@gmail.com
T. J. Harikrishnan: Department of Veterinary Parasitology,
Veterinary College and Research Institute, Namakkal, Tamil Nadu,
India; 3. Poultry Disease Diagnosis and Surveillance Laboratory,
Veterinary College and Research Institute Campus, Namakkal, Tamil
Nadu, India; hari@tanuvas.org.in
P. Srinivasan: Poultry Disease Diagnosis and Surveillance
Laboratory, Veterinary College and Research Institute Campus,
Namakkal, Tamil Nadu, India; srinipat2004@yahoo.com
G. Selvaraju: Department of Veterinary Epidemiology and
Preventive Medicine, Veterinary College and Research Institute,
Tamil Nadu Veterinary and Animal Sciences University, Namakkal,
Tamil Naud, India; g.selvaraju@tanuvas.org.in
Received: 09-04-2014, Revised: 29-05-2014, Accepted: 02-06-2014,
Published online: 03-07-2014
Corresponding author: S. Sarvanan, email:
sarvet_25@yahoo.com
Abstract
Aim: To assess the humoral
immune response of Eimeria tenella sporozoites in broiler chickens
by a developed enzyme linked immunosorbent assay (ELISA) and the
efficacy in terms of bodyweight, lesion score and oocysts
excretion in immunized broilers.
Materials and Methods: Purified live E. tenella sporozoites
were administered subcutaneously in neck region of broiler
chickens in the early life (first week) at different
concentrations. The potency of the sporozoite vaccine as assessed
by IgG levels and the performance in immunized broilers as
assessed by body weight, lesion score and oocysts excretion in
faeces after challenge with 10, 000 live E. tenella oocysts at 49
days of age were evaluated.
Results: The chickens of group (T4) immunized with 20 µg of
antigen on day 6 showed an increase in IgG levels (0.161±0.004)
two weeks post immunization (PI) peaking (0.399± 0.016) at 5 weeks
PI. The mean weekly weight gain (g) after challenge, at 56 days of
age was high in T4 (148±4.751 g) with a low mean lesion score
(2.5±0.22) and mean oocyst output (x103 oocytes per gram (OPG) in
faeces (100.3± 45.72) when compared to unimmunised infected
controls.
Conclusion: An early but partial immune response against
caecal coccidiosis could be achieved by immunization with E.
tenella specific sporozoites in chickens of less than a week old.
Moreover, the performance of immunized chickens as indicated by
weight gain, lesion score and oocyst output was found to be
superior to the unimmunized infected controls.
Keywords: bodyweight, broiler chickens, Eimeria tenella
sporozoites, ELISA, IgG levels, lesion score.
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