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Research
(Published
online: 28-03-2014)
14. Seroprevalence of caprine brucellosis
in Karnataka - D. Avinash Reddy, Gita Kumari, S.
Rajagunalan, D. K. Singh, Ashok Kumar and P. Pavan Kumar
Veterinary World, 7(3): 182-188
doi:
10.14202/vetworld.2014.182-188
Abstract
Aim: To study the
seroprevalence of caprine bruellosis in Karnataka and compare the
relative sensitivity and specificity among the different
serological tests used.
Materials and Methods: A total of 252 serum samples were
collected from the goats of Karnataka and subjected to 5 different
serological tests, i.e., Rose Bengal Plate Test (RBPT), Standard
Tube Agglutination Test (STAT), 2-mercaptoethanol test (2-MET),
Indirect ELISA (I-ELISA) and Dot-ELISA to detect the Brucella
antibodies.
Results: Test-wise, the seroprevalence in goats was 5.15%
by RBPT, 6.34% by STAT, 1.98% by 2-MET, 9.52% by I-ELISA and 7.14%
by Dot-ELISA. The prevalence of brucellosis was found to be
highest among goats of northeast Karnataka followed by northwest
Karnataka, central Karnataka and south Karnataka. I-ELISA detected
maximum number of positive samples.
Conclusions: The study used five serological tests to
determine the apparent seroprevalence of caprine brucellosis in
Karnataka. Taking I-ELISA as reference, the tests revealed the
relative sensitivity values in the following order:
Dot-ELISA>STAT>RBPT>2-MET.
Keywords: brucellosis, caprine, Karnataka, relative
sensitivity, seroprevalence.
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