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Research
(Published
online: 22-05-2014)
14. Sequencing and phylogenetic analysis of
partial CXCR2 gene of Murrah buffalo - S. A. Wani, M.
L. Sangwan, M. A. Dar, A. Kumar, M. A. Rafee and D. Baro
Veterinary World, 7(5): 342-346
doi:
10.14202/vetworld.2014.342-346
S. A. Wani:
Division of Animal Biotechnology, Indian Veterinary Research
Institute, Izatnagar - 243122, Uttar Pradesh, India
M. L. Sangwan: Department of Animal Biotechnology, Lala
Lajpat Rai University of Veterinary and Animal Science, Hisar,
Haryana, India
M. A. Dar: Department of Veterinary Physiology and
Biochemistry, Karnataka Veterinary, Animal and Fisheries Sciences
University, Hebbel, Bangalore, Karnataka, India
A. Kumar: Department of Animal Biotechnology, Lala Lajpat
Rai University of Veterinary and Animal Science, Hisar, Haryana,
India
M. A. Rafee: Division of Veterinary Surgery and Radiology,
Indian Veterinary Research Institute, Izatnagar - 243122, Uttar
Pradesh, India
D. Baro: Department of Animal Biotechnology, Lala Lajpat
Rai University of Veterinary and Animal Science, Hisar, Haryana,
India
Received: 22-02-2014, Revised: 07-04-2014, Accepted: 16-04-2014,
Published online: 22-05-2014
Corresponding author:
S. A. Wani, email: wanisajad759@gmail.com
Abstract
Aim: Present study was
carried out to sequence and phylogenetic analysis of CXCR2 gene of
Murrah buffalo.
Materials and Methods: For the present investigation, from
a group of forty eight Murrah buffaloes (Bubalus bubalis), blood
samples were collected randomly from eight animals, out of which
four were healthy and four were mastitic.
Results: The amplification of Interleukin-8B (IL-8B)
receptor gene target sequence was carried out using the primer
pair in an optimized polymerase chain reaction. Partial sequencing
of IL-8B receptor gene of Bubalus bubalis (Murrah) has been done
successfully. The sequences of IL-8B receptor gene showed 99%
homology to that of Bos indicus × Bos taurus, 98% to that of Bos
taurus, 97% to that of Ovis aries, 93% to that of Sus scrofa, 92%
to that of Equus caballus and 90% to that of Felis catus.
Conclusion: From the present study it can be concluded that
the PCR amplification procedure for target region of IL-8B
receptor gene yielding 459 bp products has been standardized,
which yielded consistent and specific amplification. Amplification
of partial IL-8B receptor gene (exon 2- 459 bp) using self
designed primers specific for cattle ortholog sequence signifies
that the locus is conserved in cattle and buffaloes. In
phylogenetic tree, the target sequence of IL-8B receptor gene of
Bubalus bubalis were found to be more closely related to Bos
indicus × Bos Taurus and Bos taurus than to Ovis aries and Sus
scrofa.
Keywords: CXCR2 gene, Murrah buffalo, phylogenetic
analysis.
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