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Research
(Published
online: 27-11-2014)
15.
Pathogens isolated
from clinical mastitis in Murrah buffaloes and their antibiogram
- Gaurav Charaya, Anshu Sharma, Ashok Kumar, Mahavir Singh and
Parveen Goel
Veterinary World, 7(11): 980-985
doi:
10.14202/vetworld.2014.980-985
Gaurav
Charaya:
Department of Veterinary Medicine, College of Veterinary Sciences,
Lala Lajpat Rai University of Veterinary and Animal Sciences,
Hisar, Haryana, India;
gcharaya9@gmail.com
Anshu
Sharma: College
Central Laboratory, College of Veterinary Sciences, Lala Lajpat
Rai University of Veterinary and Animal Sciences, Hisar, Haryana,
India;
anshusharma_dr@yahoo.com
Ashok
Kumar: Department
of Veterinary Medicine, College of Veterinary Sciences, Lala
Lajpat Rai University of Veterinary and Animal
Sciences,
Hisar, Haryana, India;
aggar2050@hotmail.com
Mahavir
Singh: College
Central Laboratory, College of Veterinary Sciences, Lala Lajpat
Rai University of Veterinary and Animal Sciences, Hisar, Haryana,
India;
drmahaviryadav@gmail.com
Parveen
Goel: Department of Veterinary Medicine, College of Veterinary
Sciences, Lala Lajpat Rai University of Veterinary and Animal
Sciences,
Hisar, Haryana, India;
parveengoel.hsr@gmail.com
Received:
21-07-2014, Revised: 14-10-2014, Accepted: 23-10-2014, Published
online: 27-11-2014
Corresponding author: Anshu
Sharma, e-mail: anshusharma_dr@yahoo.com
Abstract
Aim:
To determine the etiology of clinical mastitis in Murrah
buffaloes and to develop an antibiogram of organisms isolated.
Materials and Methods: A total of 564 quarter milk samples of
144 buffaloes suffering from clinical mastitis received in
Veterinary College Central Laboratory were inoculated on blood
agar, MacConkey’s lactose agar and sabouraud dextrose agar.
Bacteria isolated were characterized to the species level and
subjected to in-vitro antimicrobial sensitivity testing.
Results: Out of 564 quarters examined for mastitis, 320
(56.73%) quarters were found culturally positive showing isolation
of Staphylococcus aureus 140 (38.04%), Streptococcus
dysgalactiae 112 (30.43%), Streptococcus agalactiae 13
(3.53%), Escherichia coli 74 (20.10%) and Corynebacterium
pyogenes 29 (7.88%). On carrying antibiogram staphylococci and
streptococci revealed high sensitivity towards chloramphenicol,
gentamicin, amikacin and enrofloxacin. Streptococci showed high
sensitivity towards ceftriaxone and cefaperazone also. E. coli
was found highly sensitive to chloramphenicol and gentamicin.
C. pyogenes was sensitive to the majority of antibiotics.
Conclusion: S. aureus was the most predominant bacteria
isolated from mastitis cases and irrespective of the isolates
chloramphenicol was found to be most sensitive when tested
in-vitro.
Keywords: antibiogram, Escherichia coli., mastitis,
staphylococci, streptococci.
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