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Research
(Published
online: 19-10-2014)
19.
Assessment of genetic variability among Indian sheep breeds using
mitochondrial DNA cytochrome-b region - A. D. Sawaimul,
M. G. Sahare, S. Z. Ali, A. R. Sirothia and Satish Kumar
Veterinary World, 7(10): 852-855
doi:
10.14202/vetworld.2014.852-855
A. D.
Sawaimul:
Animal Biotechnology Section, Central Sheep and Wool Research
Institute, Avikanagar, Rajasthan, India;
Department of Animal Genetics and Breeding, Nagpur Veterinary
College, Nagpur, Maharashtra, India;
aviratsawaimul@gmail.com
M. G.
Sahare:
Animal Biotechnology Section, Central Sheep and Wool Research
Institute, Avikanagar, Rajasthan, India;
Department of Animal Genetics and Breeding, Nagpur Veterinary
College, Nagpur, Maharashtra, India;
drmaheshagb@gmail.com
S. Z.
Ali:
Department of Animal Genetics and Breeding, Nagpur Veterinary
College, Nagpur, Maharashtra, India;
szali@rediffmail.com
A. R.
Sirothia:
Department of Animal Genetics and Breeding, Nagpur Veterinary
College, Nagpur, Maharashtra, India;
arsirothia@gmail.com
Satish
Kumar: Animal Biotechnology Section, Central Sheep and Wool
Research Institute, Avikanagar, Rajasthan, India;
biotech.satish@gmail.com
Received:
10-06-2014, Revised: 13-09-2014, Accepted: 20-09-2014, Published
online: 19-10-2014
Corresponding author:
Satish Kumar, e-mail: biotech.satish@gmail.com
Abstract
Aim:
The present study was conducted to estimate genetic distance,
the phylogenetic relationship, and time of divergences using
mitochondrial DNA (mtDNA).
Materials and Methods: The total 216 unrelated samples were
collected from native breeding tract of six Indian sheep breeds.
The genomic DNA was isolated and screened for restriction enzyme
polymorphisms for cytochrome b (Cyt-b) region of mtDNA with seven
restriction enzymes.
Results: The genetic distance among sheep breeds was ranging
between 0.02833 and 0.0946. The phylogenetic analysis revealed
that Malpura and Chokla were found closer relationship forming
distinct cluster followed by Deccani individual were clustered
with Nellore sheep, whereas Nali and Sonadi were distant to each
other having separate cluster. Estimated divergence time among
Indian sheep breeds were ranging about 1.41-4.73 million years ago
(MYA) with an average of 3.063±0.27 MYA. It showed that Malpura
and Sonadi sheep revealed highest divergence time as 4.73 MYA
whereas Malpura and Chokla show the lowest as 1.41 MYA.
Conclusion: In conclusion, the restriction fragment length
polymorphisms-polymerase chain reaction (RFLP-PCR) of the Cyt-b
region of mtDNA is suitable and cost effective tool for estimating
the genetic variability, phylogenetic relationship, and time of
divergence among Indian sheep breeds. These findings will help to
formulate proper breeding strategies for conservation and
utilization of sheep breeds.
Keywords: genetics-diversity, mitochondrial DNA, restriction
fragment length polymorphisms-polymerase, chain reaction, sheep
breed.
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