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Research
(Published
online: 31-10-2014)
24.
Variations in free radical scavenging
activities and antioxidant responses in salivary glands of
Hyalomma anatolicum anatolicum and Hyalomma dromedarii
(Acari: Ixodidae) ticks -
Mayukh Ghosh, Nirmal Sangwan and Arun K. Sangwan
Veterinary World, 7(10): 876-881
doi:
10.14202/vetworld.2014.876-881
Mayukh
Ghosh: Department
of Veterinary Physiology and Biochemistry, College of Veterinary
Sciences, Lala Lajpat Rai University of
Veterinary
and Animal Sciences, Hisar, Haryana, India;
ghosh.mayukh87@gmail.com
Nirmal
Sangwan:
Department of Veterinary Physiology and Biochemistry, College of
Veterinary Sciences, Lala Lajpat Rai University of
Veterinary
and Animal Sciences, Hisar, Haryana, India;
nirmalsangwan@gmail.com
Arun K.
Sangwan: Department of Veterinary Parasitology, College of
Veterinary Sciences, Lala Lajpat Rai University of Veterinary and
Animal Sciences, Hisar, Haryana, India;
sangwan_arun@hotmail.com
Received:
21-06-2014, Revised: 15-09-2014, Accepted: 22-09-2014, Published
online: 31-10-2014
Corresponding author:
Nirmal Sangwan, e-mail: nirmalsangwan@gmail.com
Abstract
Aim:
Hyalomma anatolicum anatolicum and Hyalomma
dromedarii ticks are of major economic importance in the
livestock sector as the vector of tropical theileriosis causing
huge production loss, mostly in tropical countries. The release of
different reactive oxygen and nitrogen species by exogenous and
endogenous means can potentially induce oxidative damage to the
ticks during their prolonged feeding on their vertebrate hosts.
Hence, ticks need an effective free radical scavenging and
antioxidant defense system for their successful feeding of a blood
meal. Therefore, the present study was undertaken to evaluate the
interspecies variations in antioxidant response, free radical
scavenging, and anti-inflammatory activities in salivary gland
extracts (SGE) of the two species as they differ considerably in
relation to feeding behavior and host specificity.
Materials and Methods: Tick salivary glands were dissected out
under ice from semi-fed female ticks of both the species and
homogenized at low temperature to prepare SGE. SGE was stored at
−40°C for analysis of free radical scavenging activities and
antioxidant status.
Results: Significant depletion in reduced glutathione
concentrations, malondialdehyde level and elevation in free
radical scavenging activity, superoxide dismutase,
anti-inflammatory activity were found in SGE of engorging female
H. dromedarii ticks as compared to H. a. anatolicum.
Conclusion: Higher antioxidant status and free radical
scavenging activities in H. dromedarii might have enabled
these ticks to suck more blood from the host in spite of
continuous host’s immune responses. These findings about tick
biology will help in improving tick control strategies.
Keywords: anti-inflammatory, antioxidants, free radicals,
Hyalomma anatolicum anatolicum, Hyalomma dromedarii.
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