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Research
(Published
online: 09-10-2014)
5.
Prevalence of Campylobacter jejuni
and Campylobacter coli among broilers in Bareilly region
- Hina
Malik, Ashok Kumar, S. Rajagunalan, J. L. Kataria, Anjay and Swati
Sachan
Veterinary World, 7(10): 784-787
doi:
10.14202/vetworld.2014.784-787
Hina
Malik:
Division of Veterinary Public Health, Indian Veterinary Research
Institute (IVRI), Izatnagar, Uttar Pradesh, India;
hinaiqbal@rediffmail.com
Ashok
Kumar:
Division of Veterinary Public Health, Indian Veterinary Research
Institute (IVRI), Izatnagar, Uttar Pradesh, India;
ashokakt@rediffmail.com
S.
Rajagunalan:
Division of Veterinary Public Health, Indian Veterinary Research
Institute (IVRI), Izatnagar, Uttar Pradesh, India;
drgunavet@gmail.com
J. L.
Kataria:
Division of Veterinary Public Health, Indian Veterinary Research
Institute (IVRI), Izatnagar, Uttar Pradesh, India;
jluvjkat@gmail.com
Anjay:
Division of Veterinary Public Health, Indian Veterinary Research
Institute (IVRI), Izatnagar, Uttar Pradesh, India;
dranjayvet@gmail.com
Swati
Sachan: Immunology Section, Indian Veterinary Research
Institute (IVRI), Izatnagar, Uttar Pradesh, India;
swatischan@gmail.com
Received:
20-06-2014, Revised: 28-08-2014, Accepted: 04-09-2014, Published
online: 08-10-2014
Corresponding author:
Hina Malik, e-mail: hinaiqbal@rediffmail.com
Abstract
Aim:
To determine the prevalence of Campylobacter jejuni and
Campylobacter coli among broilers at the time of slaughter
in and around Bareilly, India.
Materials and Methods: A total of 100 chicken caecal samples
were screened by conventional plating in modified charcoal
cefoperazone deoxycholate agar with incubation at 42°C for 48 h
under microaerophilic conditions. The characteristic colonies were
confirmed by morphological and biochemical characteristics and
multiplex polymerase chain reaction (mPCR) assay targeting lpxA
gene.
Results: Out of 100 chicken caecal samples, 32 yielded
isolates with typical phenotypic of Campylobacter species.
The hippurate hydrolysis test found to be positive for 2 isolates,
categorized as C. jejuni and negative for 30 isolates. The
mPCR assay targeting lpxA gene also confirmed 2 (6.25%)
isolates as C. jejuni, and 30 (93.75%) isolates as C.
coli.
Conclusion: The present study showed broilers to an important
source of Campylobacter in the region with predominance of
C. coli than C. jejuni indicating a shift in the
prevalence of important species of Campylobacter. To
understand the variation in pattern of occurrence of species with
high prevalence of organisms, detail studies on the ecology of
campylobacteriosis are suggested.
Keywords: Campylobacter coli, Campylobacter jejuni,
multiplex polymerase chain reaction, lpxA gene.
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