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Research
(Published
online: 09-02-2015)
3. Effects of fetal bovine serum and estrus
buffalo serum on maturation of buffalo (Bubalus bubalis)
oocytes in vitro -
Gopal Puri, S. S. Chaudhary, V. K. Singh and
A. K. Sharma
Veterinary World, 8(2): 143-146
doi:
10.14202/vetworld.2015.143-146
Gopal Puri:
Department of Veterinary Physiology & Biochemistry, Vanbandhu
College of Veterinary Science & Animal Husbandry, Navsari
Agricultural University, Navsari - 396 450, Gujarat, India;
drgopalpuri@gmail.com
S.
S. Chaudhary:
Department of Veterinary Physiology & Biochemistry, Vanbandhu
College of Veterinary Science & Animal Husbandry, Navsari
Agricultural University, Navsari - 396 450, Gujarat, India;
sandhyachaudhary@live.com
V.
K. Singh:
Department of Veterinary Physiology & Biochemistry, Vanbandhu
College of Veterinary Science & Animal Husbandry, Navsari
Agricultural University, Navsari - 396 450, Gujarat, India;
drvksingh1981@gmail.com
A.
K. Sharma: Department of Veterinary Physiology & Biochemistry,
Vanbandhu College of Veterinary Science & Animal Husbandry,
Navsari Agricultural University, Navsari - 396 450, Gujarat,
India; drarunvet@gmail.com
Received: 04-09-2014, Revised: 22-12-2014, Accepted: 30-12-2014,
Published online: 09-02-2015
Corresponding author:
Gopal Puri, e-mail: drgopalpuri@gmail.com
Citation:
Puri G, Chaudhary SS, Singh
VK, Sharma AK (2015) Effects of fetal bovine serum and estrus
buffalo serum on maturation of buffalo (Bubalus bubalis)
oocytes in vitro, Veterinary World, 8(2): 143-146.
Abstract
Aim:
The aim was to assess the effects of fetal bovine serum (FBS)
and estrus buffalo serum (EBS) on in vitro maturation rate
of oocytes in buffalo.
Materials and Methods: Maturation rate of oocytes was assessed
in two maturation media supplemented with 20% FBS and EBS. Oocytes
maturation rate was evaluated on the basis of cumulus cell
expansion and extrusion of polar body after 24 h of in vitro
culture in CO 2 incubator.
Results: The average percentage of in vitro matured
oocytes in FBS was 83.80%, and EBS was 77.45%, respectively. The
results revealed a significant (p<0.05) increase in maturation
rate of oocytes in FBS than EBS.
Conclusion: Buffalo oocytes were better in vitro
matured in FBS than EBS.
Keywords: buffalo oocytes, estrus buffalo
serum, fetal bovine serum, in vitro maturation.
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