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Research
(Published
online: 24-01-2015)
16. Pathology and
molecular diagnosis of classical swine fever in Mizoram -
David Malswamkima, T. K. Rajkhowa, Rajesh
Chandra and T. K. Dutta
Veterinary World, 8(1): 76-81
doi:
10.14202/vetworld.2015.76-81
David
Malswamkima: Department of Veterinary Pathology, College of
Veterinary Sciences and Animal Husbandry, Central Agricultural
University, Selesih, Aizawl, Mizoram, India; tridibraj@rediffmail.com
T. K.
Rajkhowa: Department of Veterinary Pathology, College of
Veterinary Sciences and Animal Husbandry, Central Agricultural
University, Selesih, Aizawl, Mizoram, India; tridibraj09@gmail.com
Rajesh
Chandra: Department of Veterinary Pathology, College of
Veterinary Sciences and Animal Husbandry, Central Agricultural
University, Selesih, Aizawl, Mizoram, India;
rajeshchandra2k@rediffmail.com
T. K.
Dutta: Department of Veterinary Pathology, College of
Veterinary Sciences and Animal Husbandry, Central Agricultural
University, Selesih, Aizawl, Mizoram, India;
tapandutta@rediffmail.com
Received: 22-07-2014,
Revised:
05-12-2014, Accepted:
14-12-2014,
published online:
24-01-2015
Corresponding author:
T. K. Rajkhowa, e-mail: tridibraj09@gmail.com
Abstract
Aim:
Clinical histopathological and molecular diagnosis of
classical swine fever disease in pigs of Mizoram.
Materials and Methods: Totally, 31 clinically suspected pigs
from 6 districts of Mizoram were examined, and clinical symptoms
were recorded. Detailed post mortem examination of all the 31 dead
animals was conducted, and gross changes were recorded. Tissue
samples were collected for histopathological examination and
molecular diagnosis. The collected tissues (tonsil, lymph nodes,
spleen) were also processed for RNA extraction. Reverse
transcription polymerase chain reaction (RT-PCR) was performed to
detect the specific gene fragments of classical swine fever virus
(CSFV).
Results: Clinical examination of all the 31 suspected pigs
revealed typical clinical signs of CSF. All the animals also
showed typical gross and microscopic lesions of CSF. RT-PCR on
tissue samples amplified the 421bp, 449bp and 735bp region of
5ŽNCR, non-structural protein 5B and Erns
gene regions of CSFV, respectively. Nested PCR for internal region
of E2 gene also amplified the expected product of 271bp using PCR
product of whole E2 region as template DNA.
Conclusion: CSF is highly endemic disease in Mizoram. The
viral strains circulating in this region are highly virulent. The
disease can be diagnosed specifically using RT-PCR.
Keywords: classical swine fever, Mizoram, reverse
transcription polymerase chain reaction
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