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Research
(Published
online: 02-01-2015)
2.
Seroprevalence of
Mycoplasma gallisepticum antibody by ELISA and serum plate
agglutination test of laying chicken -
Md. Zulfekar Ali, Md. Mostafizer Rahman and
Shirin Sultana
Veterinary World, 8(1): 9-14
doi:
10.14202/vetworld.2015.9-14
Md.
Zulfekar Ali:
Department of Microbiology, Hajee Mohammad Danesh Science and
Technology University, Dinajpur, Bangladesh; zulfekar_vet@yahoo.com
Md.
Mostafizer Rahman:
Department of Microbiology, Hajee Mohammad Danesh Science and
Technology University, Dinajpur, Bangladesh; mostafiz_tm@yahoo.ca
Shirin
Sultana: Department of Pathology and Parasitology, Hajee
Mohammad Danesh Science and Technology University, Dinajpur,
Bangladesh;
shirin_vet@yahoo.com
Received: 29-09-2014, Revised: 01-12-2014, Accepted: 08-12-2014,
Published online: 02-01-2015
Corresponding author:
Md. Zulfekar Ali, email: zulfekar_vet@yahoo.com
Abstract
Aim:
Mycoplasma gallisepticum (MG) is important avian
pathogen responsible for chronic respiratory disease of chicken
and turkeys, which result in large economic loss for the poultry
industry. The objectives of this study were determination of
seroprevalence of MG antibody of commercial layer chicken at
laying period in selected areas of Bangladesh.
Materials and Methods: A total of 563 blood samples were
collected randomly from selected commercial layer chickens at
laying period during the period from July to December, 2013.
Indirect enzyme linked immunosorbent assay (iELISA) and serum
plate agglutination (SPA) test were performed to detect the
presence of antibodies against MG.
Results: Of 563 samples, 64.47% and 56.13% showed an overall
prevalence of MG antibodies in iELISA and SPA test respectively.
Prevalence of MG was recorded the highest (69.63%) at 50-55 weeks
of age compared with lowest (53.26%) at 56-61 weeks of age
(p<0.05). Significant (p<0.05) effect of breed were observed in
the seroprevalence of MG infection in layer birds in the present
study. The overall, 68.77%, 63.74% and 59.37% prevalence were
found respectively in sonali, ISA Brown and White leg horn. The
prevalence of MG antibodies was the highest (70.13%) in December
followed by November (68%), October (65.67%), August (63.46%),
September (58.54%) and July (51.78%) month. The seroprevalence of
MG antibodies was higher (69.63%) in most of the large flocks and
lower (56.82%) in small flocks.
Conclusion: Therefore, might be suggested that the commercial
layer farms should be routinely checked to monitor MG infection
and the reactor birds should be culled since MG organism has the
potential to transmit vertically. The correlation between MG
antibody in month and flock size was not significant (p=0.359 and
p=0.868, respectively).
Keywords: antibodies, Mycoplasma gallisepticum, layer
chickens, seroprevalence.
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