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R esearch
(Published online:
21-07-2015)
13.
Association of ATP1A1 gene
polymorphism with thermotolerance in Tharparkar and Vrindavani
cattle -
Neeraj Kashyap,
Pushpendra Kumar, Bharti Deshmukh, Sandip Bhat, Amit Kumar, Anuj
Chauhan, Bharat Bhushan, Gyanendra Singh and Deepak Sharma
Veterinary World, 8(7): 892-897
doi:
10.14202/vetworld.2015.892-897
Neeraj
Kashyap:
Division of Animal Genetics, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh, India;
neeraj.vety@gmail.com
Pushpendra Kumar:
Division of Animal Genetics, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh, India;
pushpendra64@gmail.com
Bharti
Deshmukh:
Department of Animal Genetics and Breeding, Govind Ballabh Pant
University of Agriculture and Technology,
Pantnagar,
Uttarakhand, India; bharti.vet@gmail.com
Sandip
Bhat:
Division of Animal Genetics, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh, India;
sandipivri@gmail.com
Amit
Kumar:
Division of Animal Genetics, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh, India;
vetamitchandan07@gmail.com
Anuj
Chauhan:
Division of Animal Genetics, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh, India;
anuj_vet99@rediffmail.com
Bharat
Bhushan:
Division of Animal Genetics, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh, India;
bbhushan_ivri2003@yahoo.co.in
Gyanendra Singh:
Division of Physiology and Climatology, Indian Veterinary Research
Institute, Izatnagar, Bareilly, Uttar Pradesh, India;
gyanendra@ivri.res.in
Deepak
Sharma: Dvision of Animal Genetics, Indian Veterinary Research
Institute, Izatnagar, Bareilly, Uttar Pradesh, India;
ds7758@yahoo.co.in
Received:
19-03-2015, Revised: 13-06-2015, Accepted: 20-06-2015, Published
Online: 21-07-2015
Corresponding author:
Neeraj Kashyap, e-mail: neeraj.vety@gmail.com
Citation:
Kashyap N, Kumar P,
Deshmukh B, Bhat S, Kumar A, Chauhan A, Bhushan B, Singh G, Sharma
D (2015) Association of ATP1A1 gene polymorphism with
thermo tolerance in Tharparkar and Vrindavani cattle,
Veterinary World 8(7): 892-897.
Abstract
Aim:
One of the major biochemical aspects of thermoregulation is
equilibrium of ion gradient across biological membranes. Na +/K+-ATPase,
a member of P type-ATPase family, is a major contributor to the
mechanism that actively controls crossmembrane ion gradient. Thus,
we examined ATP1A1 gene that encodes alpha-1 chain of Na+/K+-ATPase,
for genetic polymorphisms.
Materials and Methods: A total of 100 Vrindavani (composite
cross strain of Hariana x Holstein-Friesian/Brown Swiss/Jersey)
and 64 Tharparkar (indigenous) cattle were screened for genetic
polymorphism in ATP1A1 gene, using polymerase chain
reaction single-strand conformation polymorphism and DNA
sequencing. For association studies, rectal temperature (RT) and
respiration rate (RR) of all animals were recorded twice daily for
3 seasons.
Results: A SNP (C2789A) was identified in exon 17 of ATP1A1
gene. Three genotypes namely CC, CA, and AA were observed in
both, Vrindavani and Tharparkar cattle. The gene frequencies in
Tharparkar and Vrindavani for allele A were 0.51 and 0.48, and for
allele C were 0.49 and 0.52, respectively, which remained at
intermediate range. Association study of genotypes with RT and RR
in both cattle population revealed that the animals with genotype
CC exhibited significantly lower RT and higher heat tolerance
coefficient than CA and AA genotypes.
Conclusion: Differential thermoregulation between different
genotypes of ATP1A1 gene indicate that the ATP1A1
gene could be potentially contributing to thermotolerance in both,
Tharparkar, an indigenous breed and Vrindavani, a composite
crossbred cattle.
Keywords: ATP1A1 gene, cattle,
polymorphism, thermotolerance, Tharparkar, Vrindavani
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