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R esearch
(Published online:
29-07-2015)
19.
Isolation and detection of
Corynebacterium pseudotuberculosis in the reproductive organs
and associated lymph nodes of non-pregnant does experimentally
inoculated through intradermal route in chronic form -
Nur Amirah
Abdul Latif, Faez Firdaus Jesse Abdullah, Aishatu Mohammed Othman,
Adza Rina, Eric Lim Teik Chung, Mohd Zamri-Saad, Abdul Aziz
Saharee, Abdul Wahid Haron and Mohd Azmi Mohd Lila
Veterinary World, 8(7): 924-927
doi:
10.14202/vetworld.2015.924-927
Nur
Amirah Abdul Latif:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor,
Malaysia; nuramiraha.l@gmail.com
Faez
Firdaus Jesse Abdullah:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor,
Malaysia; Research Centre for Ruminant Disease, Universiti Putra
Malaysia, 43400 Serdang, Selangor, Malaysia;
jesseariasamy@gmail.com
Aishatu
Mohammed Othman:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor,
Malaysia;
aishatuothman@yahoo.co.uk
Adza
Rina:
Department of Veterinary Pathology and Microbiology, Faculty of
Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor, Malaysia;
adza_rina@yahoo.com
Eric
Lim Teik Chung:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor,
Malaysia; eric88lim@gmail.com
Mohd
Zamri-Saad:
Department of Veterinary Pathology and Microbiology, Faculty of
Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor, Malaysia;
mzamri@upm.edu.my
Abdul
Aziz Saharee:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor,
Malaysia; abdaziz@upm.edu.my
Abdul
Wahid Haron:
Department of Veterinary Clinical Studies, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor,
Malaysia; Research Centre for Ruminant Disease, Universiti Putra
Malaysia, 43400 Serdang, Selangor, Malaysia; wahidh@upm.edu.my
Mohd
Azmi Mohd Lila: Department of Veterinary Pathology and
Microbiology, Faculty of Veterinary Medicine, Universiti Putra
Malaysia,
43400 Serdang, Selangor, Malaysia;
azmi@upm.edu.my
Received:
03-02-2015, Revised: 22-06-2015, Accepted: 30-06-2015, Published
online: 29-07-2015
Corresponding author:
Faez Firdaus Jesse Abdullah, e-mail: jesseariasamy@gmail.com
Citation:
Latif NAA, Abdullah
FFJ, Othman AM, Rina A, Chung ELT, Zamri-Saad M, Saharee AA, Haron
AW, Lila MAM (2015) Isolation and detection of Corynebacterium
pseudotuberculosis in the reproductive organs and associated
lymph nodes of non-pregnant does experimentally inoculated through
intradermal route in chronic form, Veterinary World 8(7):924-927.
Abstract
Aim:
Corynebacterium pseudotuberculosis
is the etiological agent of caseous
lymphadenitis that affects sheep and goats. This study was
designed to determine the presence of the causative organism in
the female reproductive organs and associated lymph nodes in
non-pregnant does experimentally inoculated through intradermal
route in the chronic form.
Materials and Methods: 18
non-pregnant healthy Katjang does aged 2-year-old were divided
randomly into two groups. The first and second group consists of
nine non-pregnant does each and the two groups were subdivided
into three subgroups. The first group was experimentally
inoculated with 1 ml of 107cfu
of live C. pseudotuberculosis
through intradermal route, whereas the
second group was inoculated with 1 ml phosphate buffer saline (pH
7) solution intradermally. The first group were further subdivided
into three subgroups where, the first subgroup (B1) were kept for
30 days post-infection, second subgroup (B2) were kept for 60 days
post-infection, and third subgroup (B3) were kept for 90 days. The
second group was further subdivided into three subgroups (C1, C2,
and C3) where they were kept for 39, 60, and 90 days
post-infection, respectively.
Results: From this study,
there was successful isolation of C.
pseudotuberculosis from the
reproductive organs of the treatment group after 60 days
post-infection. The subgroups (B1, C1, C2, and C3) did not show
any presence of the causative organism in the reproductive organs.
The second subgroup B2 and third subgroup B3 showed positive
isolation of the causative organisms from the ovary, uterine
horns, uterus, cervix, vagina, and inguinal lymph node of the
experimental non-pregnant does.
Conclusion:
This study showed that chronic infection of
C. pseudotuberculosis via
intradermal route may cause effect toward the reproductive organs
and may be able to influence the reproductive efficiency of the
infected animals.
Keywords: Corynebacterium
pseudotuberculosis, chronic
form, reproductive organs, lymph nodes, detection.
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