Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
R esearch
(Published online:
31-07-2015)
21.
Genetic polymorphisms within exon 3 of heat shock protein 90AA1
gene and its association with heat tolerance traits in Sahiwal
cows -
Rakesh Kumar, I. D.
Gupta, Archana Verma, Nishant Verma and M. R. Vineeth
Veterinary World, 8(7): 932-936
doi:
10.14202/vetworld.2015.932-936
Rakesh
Kumar:
Division of Dairy Cattle Breeding, Indian Council of Agricultural
Research-National Dairy Research Institute, Karnal,
Haryana,
India; rakesh05vet@gmail.com
I. D.
Gupta:
Division of Dairy Cattle Breeding, Indian Council of Agricultural
Research-National Dairy Research Institute, Karnal,
Haryana,
India; idgupta1959@gmail.com
Archana
Verma:
Division of Dairy Cattle Breeding, Indian Council of Agricultural
Research-National Dairy Research Institute, Karnal,
Haryana,
India; archana.ndri@gmail.com
Nishant
Verma:
Division of Dairy Cattle Breeding, Indian Council of Agricultural
Research-National Dairy Research Institute, Karnal,
Haryana,
India; nish100verma@gmail.com
M. R.
Vineeth: Division of Dairy Cattle Breeding, Indian Council of
Agricultural Research-National Dairy Research Institute, Karnal,
Haryana,
India;
vineethmails@gmail.com
Received:
25-05-2015, Revised: 24-06-2015, Accepted: 02-07-2015, Published
online: 31-07-2015
Corresponding author:
I. D. Gupta, e-mail: idgupta1959@gmail.com
Citation:
Kumar R, Gupta ID,
Verma A, Verma N, Vineeth MR (2015) Genetic polymorphisms within
exon 3 of heat shock protein 90AA1 gene and its association
with heat tolerance traits in Sahiwal cows, Veterinary World 8(7):
932-936.
Abstract
Aim:
The present study was undertaken
to identify novel single nucleotide polymorphism (SNP) in Exon 3
of HSP90AA1 gene and to analyze their association with
respiration rate (RR) and rectal temperature (RT) in Sahiwal cows.
Materials and Methods:
The present study was carried
out in Sahiwal cows (n=100) with the objectives to identify novel
SNP in exon 3 of HSP90AA1 gene and to explore the
association with heat tolerance traits. CLUSTAL-W multiple
sequence analysis was used to identify novel SNPs in exon 3 of
HSP90AA1 gene in Sahiwal cows. Gene and genotype frequencies
of different genotypes were estimated by standard procedure
POPGENE version 1.32 (University of Alberta, Canada). The
significant effect of SNP variants on physiological parameters,
e.g. RR and RT were analyzed using the General Linear model
procedure of SAS Version 9.2.
Results:
The polymerase chain reaction
product with the amplicon size of 450 bp was successfully
amplified, covering exon 3 region of HSP90AA1 gene in
Sahiwal cows. On the basis of comparative sequence analysis of
Sahiwal samples (n=100), transitional mutations were detected at
locus A1209G as compared to
Bos taurus
(NCBI GenBank AC_000178.1). After
chromatogram analysis, three genotypes AA, AG, and GG with
respective frequencies of 0.23, 0.50, and 0.27 ascertained. RR and
RT were recorded once during probable extreme hours in winter,
spring, and summer seasons. It was revealed that significant
difference (p<0.01) among genetic variants of HSP90AA1 gene
with heat tolerance trait was found in Sahiwal cattle. The
homozygotic animals with AA genotype had lower heat tolerance
coefficient (HTC) (1.78±0.04a),
as compared to both AG and GG genotypes (1.85±0.03b
and 1.91±0.02c),
respectively. The gene and genotype frequencies for the locus
A1209G were ascertained.
Conclusions:
Novel SNP was found at the A1209G
position showed all possible three genotypes (homozygous and
heterozygous). Temperature humidity index has a highly significant
association with RR, RT, and HTC in all the seasons. Perusal of
results across different seasons showed the significant (p<0.01)
difference in RR, RT, and HTC among winter, spring, and summer
seasons. Genetic association with heat tolerance traits reveals
their importance as a potential genetic marker for heat tolerance
traits in Sahiwal cows.
Keywords:
heat
stress, heat tolerance coefficient, HSP90AA1,
polymorphisms, Sahiwal cattle.
References
1. Bernabucci, U., Lacetera, N., Baumgard, L.H., Rhoads, R.P.,
Ronchi, B. and Nardone, A. (2010) Metabolic and hormonal
adaptations to heat stress in domesticated ruminants. Animal.,
4: 1167-1183.
http://dx.doi.org/10.1017/S175173111000090X
PMid:22444615 |
|
|
2. Sharma, A.K., Rodriguez, L.A., Wilcox, C.J., Collier, R.J.,
Bachman, K.C. and Martin, F.G. (1988) Interactions of climatic
factors affecting milk yield and composition. J. Dairy Sci.,
71: 819-882.
http://dx.doi.org/10.3168/jds.S0022-0302(88)79622-8 |
|
|
3. Cavestany, D., El-Wishy, A.B. and Foote, R.H. (1985) Effect
of season and high environmental temperature on fertility of
Holstein cattle. J. Dairy. Sci., 68: 1471-1478.
http://dx.doi.org/10.3168/jds.S0022-0302(85)80985-1 |
|
|
|
4. Upadhyay, R.C. (2010) 2% Annual Milk Production Loss Due to
Global Warming: Research. Press trust of India/ New Delhi 26th
September. |
|
|
|
5. Pete, H. (2006) Improving Cow Pregnancy Success during
summer's heat Horizons. Genex Cooperative, Inc. Available
from: http://www. crinet.com. Pp -12:14. |
|
|
6. Hoffmann, I. (2010) Climate change and characterization,
breeding and conservation of animal genetic resources. Anim.
Genet., 41: 32-46.
http://dx.doi.org/10.1111/j.1365-2052.2010.02043.x
PMid:20500754 |
|
|
7. Collier, R.J., Collier, J.L., Rhoads, R.P. and Baumgard,
L.H. (2008) Invited review: Genes involved in the bovine heat
stress response. J. Dairy Sci., 91: 445-454.
http://dx.doi.org/10.3168/jds.2007-0540
PMid:18218730 |
|
|
|
8. McManus, C., Paiva, S.R., Braccini, N.J., Barcellos, J.O.
and Dallago, B.S. (2013) Adaptations of cattle to stressful
environments. In: Liu, G., editor. Cattle: Domestication,
Diseases and the Environmental. Nova Publishers, New York,
USA. p139-158. |
|
|
9. Paula-Lopes, F.F., Chase, J.R., Al-Katanani, C.C.,
Krininger, Y.M., Rivera, C.E., Tekin, R.M., Majewski, S.,
Ocon, A.C., Olson, T.A. and Hansen, P.J. (2003) Genetic
divergence in cellular resistance to heat shock in cattle:
Differences between breeds developed in temperate versus hot
climates in responses of preimplantation embryos, reproductive
tract tissues and lymphocytes to increased culture
temperatures. Anim. Reprod. Sci., 125: 285-294.
http://dx.doi.org/10.1530/rep.0.1250285 |
|
|
|
10. Hansen, P.J. (2004) Physiological and cellular adaptations
of zebu cattle to thermal stress. Int. J. Anim. Sci., 77:
36-50. |
|
|
11. Lacetera, N., Bernabucci, U., Scalia, D., Basiricò, L.,
Morera, P. and Nardone, A. (2006) Heat stress elicits
different responses in peripheral blood mononuclear cells from
Brown Swiss and Holstein cows. J. Dairy. Sci., 89: 4606-4612.
http://dx.doi.org/10.3168/jds.S0022-0302(06)72510-3 |
|
|
12. Chen, B., Zhong, D. and Monteiro, A. (2006) Comparative
genomics and evolution of the Hsp90 family of genes across all
kingdoms of organisms. BMC. Genomics., 7: 156-167.
http://dx.doi.org/10.1186/1471-2164-7-156
PMid:16780600 PMCid:PMC1525184 |
|
|
13. Xu, D., Zalmas, L.P. and Thangue, N.B. (2011) A
transcription cofactor required for the heat-shock response.
EMBO Rep., 9(7): 662-669.
http://dx.doi.org/10.1038/embor.2008.70
PMid:18451878 PMCid:PMC2475325 |
|
|
14. Beckham, J.T., Wilmink, G.J., Opalenik, S.R., Mackanos,
M.A., Abraham, A.A., Takahashi, K., Contag, C.H., Takahashi,
T. and Jansen, E.D. (2010) Microarray analysis of cellular
thermo tolerance. Laser Surg. Med., 42: 752-765.
http://dx.doi.org/10.1002/lsm.20983
PMid:21246580 |
|
|
15. Sareh, H., Tulapurkar, M.E., Shah, N.G., Singh, I.S. and
Hasday, J.D. (2011) Response of mice to continuous 5-day
passive hyperthermia resembles human heat acclimation. Cell
Stress Chaperones, 16: 297-307.
http://dx.doi.org/10.1007/s12192-010-0240-8
PMid:21080137 PMCid:PMC3077225 |
|
|
16. Gaughan, J.B., Mader, T.L., Holt, S.M., Sullivan, M.L. and
Hahn, G.L. (2010) Assessing the heat tolerance of 17 beef
cattle genotypes. Int. J. Bio. Sci., 54: 617-627.
http://dx.doi.org/10.1007/s00484-009-0233-4 |
|
|
17. Li, Q., Han, J., Du, F., Ju, Z., Huang, J., Wang, J., Li,
R., Wang, C. and Zhong, J. (2011) Novel SNPs in HSP70A1A gene
and the association of polymorphisms with thermo tolerance
traits and tissue specific expression in Chinese Holstein
cattle. Mol. Biol. Rep., 38: 2657-2663.
http://dx.doi.org/10.1007/s11033-010-0407-5
PMid:21082257 |
|
|
18. Shergojry, A.S., Ramesha, K.P., Mir, A.N. and Aarif, O.
(2011) Association of single nucleotide polymorphisms (SNPS)
Of HSP90AA1 gene with reproductive traits in Deoni cattle.
Int. J. Livest. Res., 1: 17-29.
http://dx.doi.org/10.5455/ijlr.20120204082821 |
|
|
19. Reddacliff, L.A., Beh, K., McGregor, H. and Whittington,
R.J. (2005) A preliminary study of possible genetic influences
on the susceptibility of sheep to Johne's disease. Aust. Vet.
J., 83: 435-441.
http://dx.doi.org/10.1111/j.1751-0813.2005.tb13087.x
PMid:16035186 |
|
|
20. Liu, Y., Li, D., Huixia, Li., Zhou, X. and Wang, G. (2011)
A novel SNP of the ATP1A gene is associated with heat
tolerance traits in dairy cows. Mol. Biol. Rep., 38: 83-88.
http://dx.doi.org/10.1007/s11033-010-0080-8
PMid:20336380 |
|
|
21. Hoffmann, A.A., Sorensen, J.G. and Loeschcke, V. (2003)
Adaptation to Drosophila to temperature extremes: Bringing
together quantitative and molecular approaches. J. Therm.
Biol., 28: 175-216.
http://dx.doi.org/10.1016/s0306-4565(02)00057-8 |
|
|
|
22. Sambrook, J. and Russell, D.W. (1989) Molecular cloning: A
Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold
Spring Harbor, New York. |
|
|
|
23. Benezra, M.V. (1954) A new index for measures the
adaptability of cattle to tropical condition. Proc. J. Anim.
Sci., 13: 1015. |
|
|
24. Berry, I.L., Shanklin, M.D. and Johnson, H.D. (1964) Dairy
shelter design based on milk production decline as affected by
temperature and humidity. Trans. Am. Soc. Agric. Eng., 7:
329-331.
http://dx.doi.org/10.13031/2013.40772 |
|
|
25. Thom, E.C. (1959) The discomfort index. Weather-wise, 12:
57-59.
http://dx.doi.org/10.1080/00431672.1959.9926960 |
|
|
|
26. Yeh, F.C., Yang, R.C. and Boyle, T. (1999) POPGENE VERSION
1.31: Microsoft Window-based free Software for Population
Genetic Analysis. Available from:
ftp://ftp.microsoft.com/Softlib/HPGL.EXE. |
|
|
27. Beatty, D.T., Barnes, A., Taylor, E., Pethick, D.,
McCarthy, M. and Maloney, S.K. (2006) Physiological responses
of Bos taurus and Bos indicus cattle to prolonged, continuous
heat and humidity. J. Anim. Sci., 84: 972-985.
PMid:16543576 |
|
|
28. Charoensook, R., Gatphayak, K., Sharifi, A.R.,
Chaisongkram, C., Brenig, B. and Knorr, C. (2012)
Polymorphisms in the bovine HSP90AB1 gene are associated with
heat tolerance in Thai indigenous cattle. Trop. Anim. Health.
Prod., 44(4): 921-928.
http://dx.doi.org/10.1007/s11250-011-9989-8
PMid:22008953 PMCid:PMC3289787 |
|
|
29. Deb, R., Sajjanar, B. and Singh, U. (2013) Promoter
variants at AP2 box region of Hsp70.1 affect thermal stress
response and milk production traits in frieswal cross bred
cattle. Genetics, 09: 37.
http://dx.doi.org/10.1016/j.gene.2013.09.037 |
|
|
30. Sajjanar, B., Deb, R., Singh, U., Kumar, S., Brahmane, M.,
Nirmale, A., Kumar, S. and Minhas, P. (2015) Identification of
SNP in HSP90AB1 and its association with the relative thermo
tolerance and milk production traits in indian dairy cattle.
Anim. Biotechnol., 26(1): 921-928
http://dx.doi.org/10.1080/10495398.2014.882846
PMid:25153455 |
|
|
31. Marcos-Carcavilla, A., Mutikainen, M., González, C.,
Jorge, H., Calvo, J., Kantanen, A., Nurbiy, S.,
Marzanov-María, D. and Magdalena Serrano, J.B. (2010) A SNP in
the HSP90AA1 gene 5′ flanking region is associated with the
adaptation to differential thermal conditions in the ovine
species. Cell Stress Chaperones., 15: 67-81.
http://dx.doi.org/10.1007/s12192-009-0123-z
PMid:19496025 PMCid:PMC2866970 |
|
