Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
R esearch
(Published online:
16-03-2015)
12.
Variations of motility and survival with
storage time at 4°C of epididymal spermatozoa Ouled-Djellal breed
rams in Eastern Algeria - B. Safsaf, S. Belkadi, L.
Belkacem, B. Mamache and M. Tlidjane
Veterinary World, 8(3): 326-329

doi:
10.14202/vetworld.2015.326-329
B.
Safsaf:
Department of Veterinary Science, Laboratory ESPA, Veterinary and
Agricultural Sciences Institute, Hadj Lakhdar University, Batna -
05000, Algeria;
saf2_b@yahoo.fr
S.
Belkadi:
Department of Veterinary Science, Laboratory ESPA, Veterinary and
Agricultural Sciences Institute, Hadj Lakhdar University, Batna -
05000, Algeria;
souhilabelkadi@gmail.com
L.
Belkacem:
Department of Veterinary Science, Laboratory ESPA, Veterinary and
Agricultural Sciences Institute, Hadj Lakhdar University, Batna -
05000, Algeria;
lbelkacem@yahoo.fr
B.
Mamache:
Department of Veterinary Science, Laboratory ESPA, Veterinary and
Agricultural Sciences Institute, Hadj Lakhdar University, Batna -
05000, Algeria;
mamachebakir@yahoo.fr
M.
Tlidjane: Department of Veterinary Science, Laboratory ESPA,
Veterinary and Agricultural Sciences Institute, Hadj Lakhdar
University, Batna - 05000, Algeria;
ayathir@yahoo.fr
Received: 14-11-2014, Revised: 28-01-2015, Accepted: 05-02-2015,
Published online: 16-03-2015
Corresponding author:
B. Safsaf, e-mail: saf2_b@yahoo.fr
Citation:
Safsaf B, Belkadi S,
Belkacem L, Mamache B, Tlidjane M (2015) Variations of motility
and survival with storage time at 4°C of epididymal spermatozoa
Ouled-Djellal breed rams in Eastern Algeria, Veterinary World
8(3):326-329.
Abstract
Aim:
The aim of this study was to evaluate some reproduction
performances in Ouled-Djellal rams.
Materials and Methods: This study involved genital organs
removed after slaughter from 54 rams at the municipal
slaughterhouse of Batna (East Algeria).
Results: The measurements of survival and mobility of
epididymal sperm followed at 0, 24, 48 and 72 h after collection,
revealed significant (p<0.05) to highly significant differences
(p>0.001) according to time. Thus, concerning the sperm motility
the values were 91.00±2.40%, 89.20±2.40%, 77.00±6.20% and
62.60±1.20% at 0, 24, 48 and 72 h, respectively. Indeed, in live
sperm, the viability rates were 82.15±1.48%, 77.67±1.74%,
66.56±1.95% and 52.30±1.46% at 0, 24, 48 and 72 h, respectively.
Conclusion: This study revealed that epididymal spermatozoa
stored at 04°C for 72 h kept their mobility and vitality at nearly
a half of their the original parameters.
Keywords: breed ouled-djellal, epididymal
sperm, rams, storage at 4°C
References
1. MADR (Ministry of Agriculture and Rural Development-
Algeria). Algeria Report (1). Current situation of breeding
camels in arid and semi-arid areas. In: International Workshop
on: The impact of climate change on livestock and sustainable
rangeland management in arid and semi-arid areas of North
Africa. Ouargla 21-24 November 2011. |
|
2. Mukasa-Mugerwa, E. and Ezaz, Z. (1992) Relationship of
testicular growth and size to age, body weight and onset of
puberty in Menzram lambs. Theriogenology, 38: 979-988.
http://dx.doi.org/10.1016/0093-691X(92)90172-N |
|
3. Ehling, C., Rath, D., Struckmann, C., Frenzel, A.,
Schindler, L. and Niemann, H. (2006) Utilization of
frozen-thawed epididymal ram semen to preserve genetic
diversity in Scrapie susceptible sheep breeds. Theriogenology,
66, 2160-2164.
http://dx.doi.org/10.1016/j.theriogenology.2006.07.003
PMid:16905182 |
|
4. Kaabi, M., Paz, P., Alvarez, M., Anel, E., Boixo, JC.,
Rouissi, H., Herraez, P. and Anel, L. (2003) Effect of
epididymis handling conditions on the quality of ram
spermatozoa recovered post-mortem. Theriogenology, 60(7):
1249-1259.
http://dx.doi.org/10.1016/S0093-691X(03)00139-0 |
|
5. Martinez-Pastor, F., Diaz-Corujo, AR., Anel, E., Herraez,
P., Anel, L. and de Paz, P. (2005) Post mortem time and season
alter subpopulation characteristics of Iberian red deer
epididymal sperm. Theriogenology, 64: 958-974.
http://dx.doi.org/10.1016/j.theriogenology.2005.01.003
PMid:16054499 |
|
6. Álvarez, M., Tamayo-Canul, J., Martínez-Rodrígueza, C.,
López-Uruena, E., Gomes-Alves, S., Anel, L., Martínez-Pastor,
F. and de Paz, P. (2012) Specificity of the extender used for
freezing ram sperm depends of the spermatozoa source
(ejaculate, electroejaculate or epididymis). Anim. Reprod.
Sci., 132(3-4): 145-154.
http://dx.doi.org/10.1016/j.anireprosci.2012.05.006
PMid:22673394 |
|
7. Braun, J., Sakai, M., Hochi, S. and Oguri, N. (1994)
Preservation of ejaculated and epididymal stallion spermatozoa
by cooling and freezing. Theriogenology, 41(4): 809-818.
http://dx.doi.org/10.1016/0093-691X(94)90497-7 |
|
8. Songsasen, N., Tong, J. and Leibo, S. (1998) Birth of live
mice derived by in vitro fertilization with spermatozoa
retrieved up to twenty-four hours after death. J. Exp. Zool.,
280(2): 189-196.
http://dx.doi.org/10.1002/(SICI)1097-010X(19980201)280:2<189::AID-JEZ10>3.0.CO;2-H |
|
9. Lambrechts, H., van Niekerk, F.E., Coetzer, W.A., Cloete,
S.W.P. and van der Horst, G. (1999) The effect of
cryopreservation on the survivability, viability and motility
of epididymal African buffalo (Svnceruscaffer) spermatozoa.
Theriogenology, 52: 1241-1249.
http://dx.doi.org/10.1016/S0093-691X(99)00201-0 |
|
10. Nichi, M., Rijsselare, T., Van Soom, A., De Clercq,
J.B.P., Goovaerts, I.G.F., Barnabe, V.H. and Bols, P.E.J.
(2007) Effect of bull epididymis storage conditions on
cryopreserved epididymal sperm in vitro fertility and lipid
peroxidation status. 33rdAnnual International Embryo Transfer
Society. 7-9 Jan., Kyoto, Japan. Reprod. Fertil. Dev., 19:
126.
http://dx.doi.org/10.1071/RDv19n1Ab15 |
|
11. Anel, L., Alvarez, M., Anel, E., Martinez-Pastor, F.,
Martinez, F., Chamorro, C. and de Paz, P. (2011) Evaluation of
three different extenders for use in emergency salvaging of
epididymal spermatozoa from a Cantabric brown bear. Reprod.
Domest. Anim., 46: 85-90.
http://dx.doi.org/10.1111/j.1439-0531.2010.01646.x
PMid:20546173 |
|
12. Blash, S., Melican, D. and Gavin, W. (2000)
Cryopreservation of epididymal sperm obtainedat necropsy from
goats. Theriogenology, 54: 899-905.
http://dx.doi.org/10.1016/S0093-691X(00)00400-3 |
|
13. Kundu, C.N., Chakraborty, J., Dutta, P., Bhattacharyya,
D., Ghosh, A. and Majumder, G.C. (2000) Development of a
simple sperm cryopreservation model using a chemically defined
medium and goat cauda epididymal spermatozoa. Cryobiology, 40:
117-125.
http://dx.doi.org/10.1006/cryo.2000.2230
PMid:10788311 |
|
14. Tamayo-Canul, J., Alvareza, M., López-Uruena, E., Nicolas,
M., Martinez-Pastora, F., Anel, E., Anel, L. and de Paz, P.
(2011) Undiluted or extended storage of ram epididymal
spermatozoa as alternatives to refrigerating the whole
epididymes. Anim. Reprod. Sci., 126: 76-82.
http://dx.doi.org/10.1016/j.anireprosci.2011.04.011
PMid:21601384 |
|
15. Fernández-Santos, M.R., Soler, A.J., Ramón, M.,
Ros-Santaella, J.L., Maroto-Morales, A., García-Álvarez, O.,
Bisbal, A., Garde, J.J., Coloma, M.A. and Santiago-Moreno J.
(2011) Effect of post-mortem time on post-thaw characteristics
of Spanish ibex (Capra pyrenaica) spermatozoa. Anim. Reprod.
Sci., 129(1-2): 56-66.
http://dx.doi.org/10.1016/j.anireprosci.2011.09.011
PMid:22014412 |
|
16. Guérin, Y., Locatelli, Y., Comizolli, P., Mauget, R.,
Mermillod, P., Legendre, X., Gatti, J.L. and Dacheux, J.L.
(2003) Conservation et utilisation du sperme épididymaire
d'ovins et de cervidés en insémination artificielle et
fécondation in vitro. Les Actes du BRG, 4: 173-183. |
|
17. Hishinuma, M. and Sekine, J. (2003) Evaluation of membrane
integrity of canine epididymal spermatozoa by short
hypoosmotic swelling test with ultrapure water. J. Vet. Med.
Sci., 65: 817-820.
http://dx.doi.org/10.1292/jvms.65.817
PMid:12939512 |
|
18. Martinez-Pastor, F., Guerra, C., Kaabi, M., Diaz, A.R.,
Anel, E., Herraez, P., de Paz, P. and Anel, L. (2005) Decay of
sperm obtained from epididymes of wild ruminants depending on
postmortem time. Theriogenology, 63(1): 24-40.
http://dx.doi.org/10.1016/j.theriogenology.2004.03.003
PMid:15589271 |
|
19. Varisli, O., Uguz, C., Agca, C. and Agca, Y. (2009)
Motility and acrosomal integrity comparisons between
electro-ejaculated and epididymal ram sperm after exposure to
a range of anisosmotic solutions, cryoprotective agents and
low temperatures. Anim. Reprod. Sci., 110: 256-268.
http://dx.doi.org/10.1016/j.anireprosci.2008.01.012
PMid:18294786 |
|
20. Barth, A. and Oko, R. (1989) Abnormal Morphology of Bovine
Spermatozoa. 1st ed. Iowa State University Press, Ames, Iowa. |
|
21. Eddy, E.M. (2006) Spermatozoon. In: Neill, J.D. and
editor. Knobil and Neill's Physiology of Reproduction. 3rd ed.
Elsevier, St Louis, Missouri. p3-55. |
|
22. Robaire, B., Hinton, B.T. and Orgebin-Crist, MC. (2006)
The epididymis. In: Neill, J.D. and editor. Knobil and Neill's
Physiology of Reproduction. 3rd ed. Elsevier, St Louis,
Missouri. p1071-1148. |
|
23. Martins, C.F., Driessen, K., Melo Costa, P.,
Carvalho-Neto, J.O., de Sousa, R.V., Rumpf, R. and Dode, M.N.
(2009) Recovery, cryopreservation and fertilization potential
of bovine spermatozoa obtained from epididymides stored at 5 C
by different periods of time. Anim. Reprod. Sci., 116(1-2):
50-57.
http://dx.doi.org/10.1016/j.anireprosci.2008.12.018
PMid:19181463 |
|
24. Lone, F.A., Islam, R., Khan, M.Z. and SofiK.A. (2011)
Effect of transportation temperature on the quality of cauda
epididymal spermatozoa of ram. Anim. Reprod. Sci., 123(1-2):
54-59.
http://dx.doi.org/10.1016/j.anireprosci.2010.10.012
PMid:21131148 |
|
25. Mir, S.S., Lone, F.A., Khan, M.Z., Malik, A.A., Islam R.
and Sofi K.A. (2012) Effect of cold storage period on the
quality of ram cauda epididymal spermatozoa recovered
postmortem. Turk. J. Vet. Anim. Sci., 36(6): 683-687. |
|
26. Rath, D. and Niemann, H. (1997) In vitro fertilization of
porcine oocytes with fresh and frozen-thawed ejaculated or
frozenthawed epididymal semen obtained from identical boars.
Theriogenology, 47(4): 785-793.
http://dx.doi.org/10.1016/S0093-691X(97)00034-4 |
|
27. Rickard, J.P., Pini, T., Soleilhavoup, C., Cognié, J.,
Bathgate, R., Lynch, GW., Evans, G., Maxwell, W.M., Druart, X.
and de Graaf S. (2014) Seminal plasma aids the survival and
cervical transit of epididymal ram spermatozoa. Reproduction,
148(5): 469-478.
http://dx.doi.org/10.1530/REP-14-0285
PMid:25118301 |
|
28. Hishinuma, M., Suzuki, K. and Sekine, J. (2003) Recovery
and cryopreservation of sika deer (Cervusnippon) spermatozoa
from epididymides stored at 48C. Theriogenology, 59(3-4):
813-820.
http://dx.doi.org/10.1016/S0093-691X(02)01154-8 |
|