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R esearch
(Published online:
16-03-2015)
14.
Neutrophil dynamics in the blood and milk of
crossbred cows naturally infected with
Staphylococcus aureus -
Dilip K. Swain,
Mohar Singh Kushwah, Mandheer Kaur and Ajay K. Dang
Veterinary World, 8(3): 336-345
doi:
10.14202/vetworld.2015.336-345
Dilip
K. Swain:
Division of Dairy Cattle Physiology, Lactation and
Immunophysiology Laboratory, National Dairy Research Institute,
Karnal - 132 001, Haryana, India;
dilip_swain@yahoo.com
Mohar
Singh Kushwah:
Division of Dairy Cattle Physiology, Lactation and
Immunophysiology Laboratory, National Dairy Research Institute,
Karnal - 132 001, Haryana, India;
drmoharvet@gmail.com
Mandheer Kaur:
Division of Dairy Cattle Physiology, Lactation and
Immunophysiology Laboratory, National Dairy Research Institute,
Karnal - 132 001, Haryana, India;
mandheerbiotech@yahoo.com
Ajay K.
Dang: Division of Dairy Cattle Physiology, Lactation and
Immunophysiology Laboratory, National Dairy Research Institute,
Karnal - 132 001, Haryana, India;
rajadang@gmail.com
Received:
02-11-2014, Revised: 31-01-2015, Accepted: 09-02-2015, Published
online: 16-03-2015
Corresponding author:
Parveen Kumar, e-mail: vetsparveen@gmail.com
Citation:
Swain DK, Kushwah MS,
Kaur M, Dang AK (2015) Neutrophil dynamics in the blood and milk
of crossbred cows naturally infected with Staphylococcus aureus,
Veterinary World 8(3):336-345.
Abstract
Aim:
The present study was designed
to evaluate the neutrophil dynamics in terms of the functional
competence during subclinical mastitis (SCM) and clinical mastitis
(CM).
Materials and Methods: A total
of 146 Karan fries cows were screened and were divided into three
groups as control (n=12), SCM, n=12 and CM, n=12 groups on the
basis of California mastitis test scoring, bacteriological
evaluation, gross and morphological changes in milk and by
counting milk somatic cell count (SCC). Both blood and milk
polymorphonuclear neutrophils (PMNs) were isolated in the study.
Phagocytic activity (PA) was studied by spectrophotometrically;
neutrophil extracelluar traps (NETs) were studied by scanning
electron microscopy (SEM); CD44 was quantified by flow cytometry
and apoptosis was studied by fluorescent microscopy.
Results: Significantly
(p<0.05) higher SCC, PA was found in milk of CM cows as compared
to SCM and control cows. Significantly lower (p<0.05) apoptosis
was observed in PMNs isolated from both blood and milk of CM group
of cows when compared to control and SCM group. The milk
neutrophils of CM group of cows formed NETs as evidenced from the
SEM images. Surface expression of CD44 revealed a significantly
(p<0.05) lower expression in milk neutrophils of CM group of cows
when compared to SCM and control group of cows.
Conclusion: The study
indicated a positive correlation between delayed neutrophil
apoptosis, persistent staying of neutrophils at the site of
infection along with formation of NETs as the strategies to fight
against the pathogens in the udder during
Staphylococcal mastitis.
The study forms a strong base
for future molecular research in terms of neutrophil recruitment
and neutrophil removal from the site of infection.
Keywords:
apoptosis, CD44, mastitis, neutrophil, neutrophil extracelluar
traps, Staphylococcus aureus.
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