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(Published online: 23-03-2015)
21.
Polymorphism and association of growth
hormone gene with growth traits in Sirohi and Barbari breeds of
goat - Praduman Pal Singh, Sateyndra Singh Tomar, Mohan
Singh Thakur and Amit Kumar
Veterinary World, 8(3): 382-387
doi:
10.14202/vetworld.2015.382-387
Praduman Pal Singh:
Department of Animal Genetics and Breeding, Veterinary College,
Nanji Deshmukh Veterinary Science University, Jabalpur, Madhya
Pradesh, India;
pradumanpalsingh@gmail.com
Sateyndra Singh Tomar:
Department of Animal Genetics and Breeding, Veterinary College,
Nanji Deshmukh Veterinary Science University, Mhow, Madhya
Pradesh, India;
dr_tomarss@rediffmail.com
Mohan
Singh Thakur:
Department of Animal Genetics and Breeding, Veterinary College,
Nanji Deshmukh Veterinary Science University,
Jabalpur,
Madhya Pradesh, India; drmohansingh@gmail.com
Amit
Kumar: Department of Animal Genetics and Breeding, Veterinary
College, Nanji Deshmukh Veterinary Science University,
Jabalpur,
Madhya Pradesh, India;
jha.amit002@gmail.com
Received: 22-10-2014, Revised: 02-02-2015, Accepted: 09-02-2015,
Published online: 23-03-2015
Corresponding author:
Amit Kumar, e-mail: jha.amit002@gmail.com
Citation:
Singh PP, Tomar SS,
Thakur MS, Kumar A (2015) Polymorphism and association of growth
hormone gene with growth traits in Sirohi and Barbari breeds of
goat, Veterinary World 8(3):382-387.
Abstract
Aim:
The aim was to study the polymorphism of exon 2 and exon 3 of
growth hormone (GH) gene, to test the polymorphic variants for
Hardy–Weinberg equilibrium and to investigate association of these
polymorphisms with chest girth and paunch girth in Sirohi and
Barbari breeds of goat.
Materials and Methods: A total of 80 kids involving forty each
of Sirohi and Barbari breeds of goat were included in the study. A
good quality genomic DNA isolated from the whole blood using
standard protocol were used for polymerase chain reaction (PCR)
amplification and products obtained on restriction digestion of
amplicon with enzyme HaeIII were separated on 2% agarose gel, and
documented in a gel doc system. The chest girth and paunch girth
of kids at birth and weekly intervals up to 4 weeks of age and
subsequently at 2 months, 3 months and 6 months of age were
recorded. Allele frequency and genotype distribution of
polymorphism were tested for Hardy–Weinberg equilibrium by program
me Genepop package. Association between different genetic variants
on chest girth and paunch girth were analyzed by least squares
analysis employing suitable statistical model.
Results: The PCR product of genomic DNA isolated from kids of
Sirohi and Barbari breeds of goat on digestion with the
restriction enzyme HaeIII revealed two genotypic variants viz.,
AB and BB. None of the two breeds was in Hardy–Weinberg
equilibrium for these variants. The least squares analysis of
variance revealed non-significant effect of GH genotype and breed
× genotype interaction on chest girth and paunch girth from birth
to 180 days of age. The effect of breed was highly significant
(p<0.01) at all ages.
Conclusion: The present study showed that both the breeds were
polymorphic at the exon 2 and exon 3 loci of GH gene under study
with respect to HaeIII restriction endonuclease. None of the
breeds was in Hardy–Weinberg equilibrium for this region of GH
gene. In the present study, no significant association between GH
genotype and chest girth and paunch girth could be established but
comparatively higher chest girth and paunch girth were observed
for AB genotype across the breeds.
Keywords: Barbari, growth hormone gene,
goat, polymorphism, polymerase chain reaction-restriction
fragment length polymorphism, Sirohi.
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