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R esearch
(Published online: 26-03-2015)
24.
Typing of Staphylococcus aureus
obtained from mastitic milk of cattle and buffalo on the basis of
two virulence-associated genes (spa and clfA) -
Rahul
Yadav, Sandeep Kumar Sharma, Jyotika Yadav and Anil Kumar Kataria
Veterinary World, 8(3): 398-402
doi:
10.14202/vetworld.2015.398-402
Rahul
Yadav:
Department of Veterinary Microbiology and Biotechnology, College
of Veterinary and Animal Sciences, Rajasthan University
of Veterinary and Animal science, Bikaner, Rajasthan, India;
drrahul16889@gmail.com
Sandeep
Kumar Sharma:
Department of Veterinary Microbiology and Biotechnology. Post
Graduate Institute of Veterinary Education and Research,
Rajasthan University of Veterinary and Animal science,
Jaipur, Rajasthan,
India;
drsharmask01@hotmail.com
Jyotika
Yadav:
College of Veterinary and Animal Sciences, Hisar, Lala Lajpat Rai
University of Veterinary & Animal Sciences, Hisar, Haryana, India;
drjyotika2910@gmail.com
Anil
Kumar Kataria: Department of Veterinary Microbiology and
Biotechnology, College of Veterinary and Animal Sciences, Rajasthan
University of Veterinary and Animal science, Bikaner, Rajasthan,
India;
akkataria1@rediffmail.com
Received:
01-01-2015, Revised: 10-02-2015, Accepted: 16-02-2015, Published
online: 26-03-2015
Corresponding author:
Rahul Yadav, e-mail: drrahul16889@gmail.com
Citation:
Rahul Yadav, Sandeep
Kumar Sharma, Jyotika Yadav and Anil Kumar Kataria (2015) Typing
of Staphylococcus aureus obtained from mastitic milk of
cattle and buffalo on the basis of two virulence-associated genes
(spa and clfA), Veterinary World 8(3):398-402.
Abstract
Aim:
The present study was undertaken to type Staphylococcus
aureus isolates from cattle and buffalo mastitic milk on the
basis of spa (X-region) and clfA genes, both
responsible for producing virulence factors.
Materials and Methods: In the present investigation S.
aureus isolates were isolated as per standard protocols.
Typing of S. aureus was carried out by molecular detection
of spa and clfA gene by polymerase chain reaction.
Results: All the 32 isolates from cattle
(16) and buffalo (16) were divisible into seven spa types
with amplicon sizes ranging between 120 and 380bp. The cattle
isolates produced seven different spa amplicons of 120,
150, 200, 250, 280, 300, and 330 bp with 3, 4, 6, 8, 10, 11 and 12
number of tandem repeats, respectively whereas buffalo isolates
were divisible into five spa types with amplicons of 150,
200, 250, 330 and 380 bp having calculated number of repeats of 5,
7, 9, 12, and 14, respectively. Of the total isolates, 24 were
considered pathogenic on the basis of more than seven number of
tandem repeats. In the present investigation, clfA gene was
amplified in 27 isolates from cattle and buffalo producing two
different amplicons of 900 and 1000 bp sizes showing polymorphism.
The most (71.80%) of the isolates produced amplicons of 900 bp
while amplicon size of 1000 bp was produced by four (12.5%) of the
isolates.
Conclusion: The presence of these genes with a wide degree of
polymorphism confirmed the pathogenic potential of S. aureus
and their association with clinical manifestations in mastitis
among cattle and buffalo.
Keywords: buffalo, cattle, clfA gene,
mastitis, Staphylococcus aureus, spa (X-region) gene.
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