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R esearch
(Published online:
27-05-2015)
20.
Isolation and identification of Salmonella from
diarrheagenic infants and young animals, sewage waste and fresh
vegetables -
Amruta Nair, T.
Balasaravanan, S. V. S Malik, Vysakh Mohan, Manesh Kumar, Jess
Vergis and Deepak B. Rawool
Veterinary World, 8(5): 669-673
doi:
10.14202/vetworld.2015.669-673
Amruta
Nair:
Division of Veterinary Public Health, Indian Veterinary Research
Institute, Bareilly, Uttar Pradesh, India; amrutanairk@gmail.com
T.
Balasaravanan:
Department of Biotechnology, Nehru Arts & Science College,
Bharathiar University, Coimbatore, Tamil Nadu, India; balsarvan@rediffmail.com
S. V. S
Malik:
Division of Veterinary Public Health, Indian Veterinary Research
Institute, Bareilly, Uttar Pradesh, India; svsmalik@gmail.com
Vysakh
Mohan:
Division of Veterinary Public Health, Indian Veterinary Research
Institute, Bareilly, Uttar Pradesh, India; vysakhmohanvet@gmail.com
Manesh
Kumar:
Division of Veterinary Public Health, Indian Veterinary Research
Institute, Bareilly, Uttar Pradesh, India; vetmanesh@gmail.com
Jess
Vergis:
Division of Veterinary Public Health, Indian Veterinary Research
Institute, Bareilly, Uttar Pradesh, India;
itzjessvergis@gmail.com
Deepak
B. Rawool: Division of Veterinary Public Health, Indian
Veterinary Research Institute, Bareilly, Uttar Pradesh, India;
deepak.rawool@yahoo.com
Received:
13-02-2015, Revised: 23-04-2015, Accepted: 29-04-2015, Published
online: 27-05-2015
Corresponding author:
Amruta Nair, e-mail: amrutanairk@gmail.com
Citation:
Nair A, Balasaravanan
T, Malik SVS, Mohan V, Kumar M, Vergis J, Rawool DB (2015)
Isolation and identification of Salmonella from
diarrhoeagenic infants and young animals, sewage waste and fresh
vegetables, Veterinary World 8(5):669-673.
Abstract
Aim:
This study was carried out to determine the prevalence,
distribution, and identification of Salmonella serotypes in
diarrheagenic infants and young animals, including sewage waste
and fresh vegetables.
Materials and Methods: A total of 550 samples were processed
for the isolation of Salmonella spp., using standard
microbiological and biochemical tests. Further polymerase chain
reaction (PCR) detection of Salmonella genus was carried
out using self-designed primers targeting invA gene and
thereafter identification of important serotypes namely
Salmonella Enterica serovar Typhimurium, Salmonella
Enterica serovar Enteritidis, Salmonella Enterica serovar
Typhi was performed using published standardized multiplex PCR.
Results: An overall low prevalence of 2.5% (14/550) was
observed. The observed prevalence of Salmonella spp. in
diarrheagenic infants was 1.2% (05/400), diarrheagenic young
animals 4% (02/50), sewage waste 10% (05/50), and fresh vegetables
4% (02/50), respectively. In diarrheagenic infants, of the five
Salmonella isolates identified, two were Salmonella
Typhimurium, two Salmonella Enteritidis, and one was
unidentified and hence designated as other Salmonella
serovar. All the Salmonella isolates identified from
diarrheagenic young animals and sewage waste belonged to other
Salmonella serovar, whereas, of the two isolates recovered
from fresh vegetables, one was identified as other Salmonella
serovar, and one as Salmonella Typhimurium,
respectively.
Conclusion: Isolation of Salmonella spp. especially
from sewage waste and fresh vegetable is a matter of great concern
from public health point of view because these sources can
accidentally serve as a potential vehicle for transmission of
Salmonella spp. to animals and human beings.
Keywords: inv A, isolation, multiplex
polymerase chain reaction, Salmonella.
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